Secondary Antibodies

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Human IgG (H+L chain) chicken polyclonal antibody, Aff - Purified

Applications ELISA.
Western Blot (1/1000).
Immunocytochemistry.
Immunohistochemistry (1/250).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose.
Reactivities Human
Immunogen Purified, non-denatured Human IgG (containing both heavy and light chains).
Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Human IgG's were affinity-purified over a column conjugated with purified Human IgG (containing both heavy and light chains).

Rabbit IgG (H+L chain) chicken polyclonal antibody, Aff - Purified

Applications ELISA.
Western Blot (1/1000).
Immunocytochemistry.
Immunohistochemistry (1/250).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose.
Reactivities Rabbit
Immunogen Purified, non-denatured Rabbit IgG (containing both heavy and light chains).
Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Rabbit IgG's were affinity-purified over a column conjugated with purified Rabbit IgG (containing both heavy and light chains).

Goat IgG (H+L chain) chicken polyclonal antibody, Aff - Purified

Applications ELISA.
Western Blot (1/1000).
Immunohistochemistry (1/250).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose.
Reactivities Goat
Immunogen Purified, non-denatured Goat IgG (containing both heavy and light chains) emulsified in Freund's adjuvant.
Production. After multiple injections, eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-goat IgG's were affinity-purified over a column conjugated with purified Goat IgG (whole protein).

Rabbit IgG (H+L chain) chicken polyclonal antibody, FITC

Applications Immunocytochemistry.
Immunohistochemistry (1/250).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and for non-specific binding using Cryostat sections of unfixed embryonic chick tissues.
Reactivities Rabbit
Conjugation FITC
Immunogen Purified, non-denatured Rabbit IgG (containing both heavy and light chains).
Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Rabbit IgG's were affinity-purified over a column conjugated with purified Rabbit IgG (containing both heavy and light chains) and then conjugated with Fluorescein isothiocyanate.

Goat IgG (H+L chain) chicken polyclonal antibody, FITC

Applications Immunocytochemistry (1/1000).
Immunohistochemistry (1/250).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and for non-specific binding using Cryostat sections of unfixed embryonic chick tissues.
Reactivities Goat
Conjugation FITC
Immunogen Purified, non-denatured Goat IgG (containing both heavy and light chains) emulsified in Freund's adjuvant.
Production. After multiple injections, eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-goat IgG's were affinity-purified over a column conjugated with purified Goat IgG (containing both heavy and light chains) and then conjugated
with Fluorescein isothiocyanate.

Human IgG (H+L chain) chicken polyclonal antibody, FITC

Applications Immunocytochemistry.
Immunohistochemistry (1/250).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and for non-specific binding using Cryostat sections of unfixed embryonic chick tissues.
Reactivities Human
Conjugation FITC
Immunogen Purified, non-denatured Human IgG (containing both heavy and light chains).
Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Human IgG's were affinity-purified over a column conjugated with purified Human IgG (containing both heavy and light chains) and then conjugated with Fluorescein isothiocyanate.

Mouse IgG (H+L chain) chicken polyclonal antibody, FITC

Applications Immunocytochemistry.
Immunohistochemistry (1/250).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and for non-specific binding using Cryostat sections of unfixed embryonic chick tissues.
Reactivities Mouse
Conjugation FITC
Immunogen Purified, non-denatured Mouse IgG (containing both heavy and light chains).
Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Mouse IgG's were affinity-purified over a column conjugated with purified Mouse IgG (containing both heavy and light chains) and conjugated to Fluorescein Isothiocyanate.

Mouse IgG (H+L chain) chicken polyclonal antibody, TRITC

Applications Immunomicroscopy.
Flow Cytometry or FACS analysis.
Conjugation TRITC
Immunogen Mouse IgG whole molecule.