Secondary Antibodies

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Goat IgG (H+L chain) chicken polyclonal antibody, HRP

Applications ELISA: a 1/75,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate.
Western Blot (1/5000).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in Agarose gels and ELISA analysis.
Reactivities Goat
Conjugation HRP
Immunogen Purified, non-denatured Goat IgG (containing both heavy and light chains) emulsified in Freund's adjuvant.
Production: After multiple injections, eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-goat IgG's were affinity-purified over a column conjugated with purified Goat IgG (containing both heavy and light chains) and then conjugated with Horseradish Peroxidase.

Human IgG (H+L chain) chicken polyclonal antibody, Aff - Purified

Applications ELISA.
Western Blot (1/1000).
Immunocytochemistry.
Immunohistochemistry (1/250).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose.
Reactivities Human
Immunogen Purified, non-denatured Human IgG (containing both heavy and light chains).
Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Human IgG's were affinity-purified over a column conjugated with purified Human IgG (containing both heavy and light chains).

Rabbit IgG (H+L chain) chicken polyclonal antibody, Aff - Purified

Applications ELISA.
Western Blot (1/1000).
Immunocytochemistry.
Immunohistochemistry (1/250).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose.
Reactivities Rabbit
Immunogen Purified, non-denatured Rabbit IgG (containing both heavy and light chains).
Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Rabbit IgG's were affinity-purified over a column conjugated with purified Rabbit IgG (containing both heavy and light chains).

Goat IgG (H+L chain) chicken polyclonal antibody, Aff - Purified

Applications ELISA.
Western Blot (1/1000).
Immunohistochemistry (1/250).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose.
Reactivities Goat
Immunogen Purified, non-denatured Goat IgG (containing both heavy and light chains) emulsified in Freund's adjuvant.
Production. After multiple injections, eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-goat IgG's were affinity-purified over a column conjugated with purified Goat IgG (whole protein).

Human IgG (H+L chain) chicken polyclonal antibody, HRP

Applications ELISA: a 1/300,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethyl-benzidine as substrate.
Western Blot (1/5000).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and ELISA analysis.
Reactivities Human
Conjugation HRP
Immunogen Purified, non-denatured Human IgG (containing both heavy and light chains).
Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Human IgG's were affinity-purified over a column conjugated with purified Human IgG (containing both heavy and light chains) and then conjugated with Horseradish Peroxidase.

Mouse IgG (H+L chain) chicken polyclonal antibody, HRP

Applications ELISA: a 1/70,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate.
Western Blot: 1/200 -1/5000.
Immunocytochemistry.
Immunohistochemistry: 1/250 - 1/5000.
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose gels and ELISA analysis.
Reactivities Mouse
Conjugation HRP
Immunogen Purified, non-denatured Mouse IgG (containing both heavy and light chains).
Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Mouse IgG's were affinity-purified over a column conjugated with purified Mouse IgG (containing both heavy and light chains) and conjugated to Horseradish peroxidase.

Rabbit IgG (H+L chain) chicken polyclonal antibody, HRP

Applications ELISA: a 1/20,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate.
Western Blot (1/5000).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose gels and ELISA analysis.
Reactivities Rabbit
Conjugation HRP
Immunogen Purified, non-denatured Rabbit IgG (containing both heavy and light chains).
Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Rabbit IgG's were affinity-purified over a column conjugated with purified Rabbit IgG (containing both heavy and light chains) and then conjugated to Horseradish Peroxidase.

Mouse IgG (H+L chain) chicken polyclonal antibody, Aff - Purified

Applications ELISA : 1:20,000 - 1:100,000
Western Blot : 1:2,000 - 1:10,000
Immunohistochemistry: 1:1,000 - 1:5,000
Reactivities Mouse
Immunogen Mouse IgG whole molecule