Secondary Antibodies

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Goat IgG (H+L chain) Chicken Polyclonal Antibody
AP31796HR-N

USD 260.00

2 Weeks

Goat IgG (H+L chain) chicken polyclonal antibody, HRP

Applications ELISA: a 1/75,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate.
Western Blot (1/5000).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in Agarose gels and ELISA analysis.
Reactivities Goat
Conjugation HRP
Immunogen Purified, non-denatured Goat IgG (containing both heavy and light chains) emulsified in Freund's adjuvant.
Production: After multiple injections, eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-goat IgG's were affinity-purified over a column conjugated with purified Goat IgG (containing both heavy and light chains) and then conjugated with Horseradish Peroxidase.
Human IgG (H+L chain) Chicken Polyclonal Antibody
AP31797HR-N

USD 260.00

2 Weeks

Human IgG (H+L chain) chicken polyclonal antibody, HRP

Applications ELISA: a 1/300,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethyl-benzidine as substrate.
Western Blot (1/5000).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and ELISA analysis.
Reactivities Human
Conjugation HRP
Immunogen Purified, non-denatured Human IgG (containing both heavy and light chains).
Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Human IgG's were affinity-purified over a column conjugated with purified Human IgG (containing both heavy and light chains) and then conjugated with Horseradish Peroxidase.
Mouse IgG (H+L chain) Chicken Polyclonal Antibody
AP31798HR-N

USD 250.00

2 Weeks

Mouse IgG (H+L chain) chicken polyclonal antibody, HRP

Applications ELISA: a 1/70,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate.
Western Blot: 1/200 -1/5000.
Immunocytochemistry.
Immunohistochemistry: 1/250 - 1/5000.
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose gels and ELISA analysis.
Reactivities Mouse
Conjugation HRP
Immunogen Purified, non-denatured Mouse IgG (containing both heavy and light chains).
Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Mouse IgG's were affinity-purified over a column conjugated with purified Mouse IgG (containing both heavy and light chains) and conjugated to Horseradish peroxidase.
Rabbit IgG (H+L chain) Chicken Polyclonal Antibody
AP31799HR-N

USD 260.00

2 Weeks

Rabbit IgG (H+L chain) chicken polyclonal antibody, HRP

Applications ELISA: a 1/20,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate.
Western Blot (1/5000).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose gels and ELISA analysis.
Reactivities Rabbit
Conjugation HRP
Immunogen Purified, non-denatured Rabbit IgG (containing both heavy and light chains).
Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Rabbit IgG's were affinity-purified over a column conjugated with purified Rabbit IgG (containing both heavy and light chains) and then conjugated to Horseradish Peroxidase.
Human IgG (H+L chain) Chicken Polyclonal Antibody
R1332HRP

USD 290.00

3 Days

Human IgG (H+L chain) chicken polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1/1,000-1/5,000, Western or Dot blot), ELISA (1/10,000-1/40,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Conjugation HRP
Immunogen Human IgG whole molecule
Mouse IgG (H+L chain) Chicken Polyclonal Antibody
R1347HRP

USD 290.00

3 Days

Mouse IgG (H+L chain) chicken polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 20,000-1/100,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Conjugation HRP
Immunogen Mouse IgG whole molecule
Rabbit IgG (H+L chain) Chicken Polyclonal Antibody
R1363HRP

USD 290.00

3 Days

Rabbit IgG (H+L chain) chicken polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/140,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Conjugation HRP
Immunogen Rabbit IgG whole molecule
Rat IgG (H+L chain) Chicken Polyclonal Antibody
R1370HRP

USD 290.00

3 Days

Rat IgG (H+L chain) chicken polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:2,000 to 1:12,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Rat IgG whole molecules.