Secondary Antibodies

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Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red

Mouse IgG + IgM (H+L chain), adsorbed goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot, 1/500-1/2,500), ELISA (1/3,000-1/12,000) and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Conjugation AP

Mouse IgG + IgM (H+L chain), adsorbed goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting (Western Blot, 1:2,000-1:10,000), ELISA (1:20,000-1:100,000), Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Conjugation Biotin

Mouse IgG + IgM (H+L chain), adsorbed goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy (1:500-1:2,500) and Flow cytometry (1:2,000-1:10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation FITC

Mouse IgG + IgM (H+L chain), adsorbed goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/40,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Conjugation HRP

Mouse IgG + IgM (H+L chain), adsorbed goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/100,000
Western Blot: 1/5,000-1/20,000
Immunohistochemistry: 1/2,000-1/10,000

Mouse IgG + IgM (H+L chain), adsorbed goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy (1:500-1:2,500) and Flow cytometry (1:2,000-1:10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red

Mouse IgG (H+L chain) goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy (1/500-1/2,500) and Flow cytometry or FACS analysis (1/2,000-1/10,000) as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation FITC

Mouse IgG (H+L chain) goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy or Immunofluorescence and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red

Rat IgG (H+L chain) goat polyclonal antibody, HRP

Applications Immunoblotting (Western or Dot blot), ELISA, immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/300,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/1,500.
Conjugation HRP

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, AP

Applications Suitable for immunoblotting (western or dot blot, 1:500- 1:2,000), ELISA (1:2,000-1:8,000) and Immunohistochemistry (1:200- 1:1,000) as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Conjugation AP

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Biotin

Applications Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Chicken IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:20,000 to 1:80,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Conjugation FITC

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Texas Red

Applications Suitable for Imunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red

Hamster IgG goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 µg of Hamster IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:65,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP

Mouse IgG (H+L chain), adsorbed goat polyclonal antibody, PE

Applications Flow Cytometry: Use 50 µl of 1/10 diluted antibody to label 10e6 cells in 100 µl.
Reactivities Mouse
Conjugation PE

Mouse IgM (Mu chain specific), adsorbed goat polyclonal antibody, Purified

Applications ELISA: 5µg/ml as coating antibody.
Reactivities Mouse

Rat IgG (H+L chain), F(ab)2 Fragment, adsorbed goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/25 - 1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivities Rat
Conjugation FITC

Rat IgG (H+L chain), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Applications Flow cytometry (1/5 - 1/10): use 50 µl of the suggested working dilution to label 10e6 cells in 100 µl.
Reactivities Rat
Conjugation PE

Human IgA + IgG + IgM goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of 0.1-0.5 µg/ml diluted antibody to label 106 cells in 100 µl.
Reactivities Human
Conjugation FITC

Hamster IgG (adsorbed) goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/50; Use 50µl of the suggested working dilution to label 1 x 10e6 cells in 100µl.
Reactivities Hamster
Conjugation FITC

Mouse IgG (H+L chain), adsorbed goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 50µl of 1/50-1/200 diluted antibody to label 106 cells in 100 µl.
Reactivities Mouse
Conjugation FITC

Canine IgM goat polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Reactivities Canine
Conjugation AP

Equine IgA goat polyclonal antibody, HRP

Applications ELISA: 1/10,000 - 1/100,000.
Reactivities Equine
Conjugation HRP

Equine IgG goat polyclonal antibody, HRP

Applications ELISA: 1/10,000 - 1/100,000.
Reactivities Equine
Conjugation HRP

Equine IgM goat polyclonal antibody, HRP

Applications ELISA: 1/10,000 - 1/100,000.
Reactivities Equine
Conjugation HRP

Feline IgA goat polyclonal antibody, HRP

Applications ELISA: 1/10,000 - 1/100,000.
Reactivities Feline
Conjugation HRP

Feline IgG (Fc specific) goat polyclonal antibody, HRP

Applications ELISA: 1/10,000.
Reactivities Feline
Conjugation HRP

Porcine IgG F(c) specific goat polyclonal antibody, HRP

Applications ELISA: 1/10,000-1/100,000.
Reactivities Porcine
Conjugation HRP

Chicken IgM goat polyclonal antibody, HRP

Applications ELISA (1/10,000-1/100,000).
Reactivities Chicken
Conjugation HRP

Chicken IgA goat polyclonal antibody, HRP

Applications ELISA (1/10,000-1/100,000).
Reactivities Chicken
Conjugation HRP

Chicken IgG / Chicken IgY (Fc specific) goat polyclonal antibody, HRP

Applications ELISA: 1/10,000-1/100,000.
Reactivities Chicken
Conjugation HRP

Porcine IgG (H+L chain) goat polyclonal antibody, HRP

Applications ELISA: 1:10,000 - 1:100,000.
Reactivities Porcine
Conjugation HRP

Porcine IgG (H+L chain) goat polyclonal antibody, Aff - Purified

Applications ELISA: 1:100 - 1:10,000.
Reactivities Porcine

Canine IgE goat polyclonal antibody, FITC

Applications Flow Cytometry: 1:200 - 1:2,000.
Reactivities Canine
Conjugation FITC

Canine IgG1 goat polyclonal antibody, FITC

Applications Flow Cytometry: 1:200 - 1:2000.
Reactivities Canine
Conjugation FITC

Mouse lambda light chain goat polyclonal antibody, AP

Applications ELISA (1/1,000-1/2,000).
Reactivities Mouse
Conjugation AP

Mouse lambda light chain goat polyclonal antibody, Biotin

Applications ELISA: 1/1000 - 1/2000.
Reactivities Mouse
Conjugation Biotin

Mouse lambda light chain goat polyclonal antibody, HRP

Applications ELISA (1/4,000-1/8,000).
Reactivities Mouse
Conjugation HRP

FITC Conjugated Goat Anti-human IgM

Applications FC: 1:100; IF/ICC: 1:32-64; IHC: 1:32-64
Reactivities Human IgM
Conjugation FITC