Secondary Antibodies

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Canine IgE mouse monoclonal antibody, clone E6-2A1, Purified

Applications Western Blot.
ELISA: The antibody may be used as a Capture antibody in ELISA in combination with SM1499B as detecting antibody.
Reactivities Canine

Human IgG (heavy chain) mouse monoclonal antibody, clone SPM556, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human

Human IgG (heavy chain) mouse monoclonal antibody, clone SPM556, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human

IGHG1 (heavy chain) mouse monoclonal antibody, clone IG266, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human

IGHG1 (heavy chain) mouse monoclonal antibody, clone IG266, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human

Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, Biotin

Applications Flow Cytometry: ≤ 1 µg/106 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells.
ELISA: 1/5,000-1/10,000.
Reactivities Rat
Conjugation Biotin

Human IgD (Delta chain specific) mouse monoclonal antibody, clone IADB6, Purified

Applications Indirect Immunofluorescent staining of IgD+ human B lymphocytes: < / = 1 µg/10e6 cells.
ELISA. 1 ug/mL
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry.
Reactivities Human

Human IgE (Epsilon chain specific) mouse monoclonal antibody, clone HP6029, Purified

Applications ELISA: Purified (UNLB) antibody ≤1 ug/mL.
Western blotting
Dot- and slot-immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Ffrozen Sections.
Reactivities Human

Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, Purified

Applications Flow Cytometry.
Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells.
ELISA.
Second step reagent for rat IgG2c Monoclonal Antibodies.
Reactivities Rat

Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, PE

Applications Flow Cytometry: FITC and BIOT conjugates ≤1 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells.
ELISA.
Reactivities Rat
Conjugation PE

Human IgG1 (Fc specific) mouse monoclonal antibody, clone 8c/6-39 (HP6091), Purified

Applications ELISA.
Immunodiffusion.
Immunohistochemistry on Frozen Sections.
Not suitable for Paraffin Sections.
Reactivities Human

Human IgE (Fc specific) mouse monoclonal antibody, clone E411

Reactivities Human

Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, Cy5

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells.
Reactivities Rat
Conjugation Cy5

Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, FITC

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells.
Reactivities Rat
Conjugation FITC

Human IgD (Delta chain specific) mouse monoclonal antibody, clone IADB6, Biotin

Applications Indirect Immunofluorescent staining of IgD+ human B lymphocytes: ≤ 1 µg/106 cells.
ELISA: 1/5,000-1/10,000.
Reactivities Human
Conjugation Biotin

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 9A6, Purified

Applications ELISA.
Immunoblotting.
Reactivities Chicken

Human IgE (C epsilon 3 specific) (in vitro) mouse monoclonal antibody, clone 4F4cc

Reactivities Human

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 52G1

Reactivities Human

Human IgA + IgG + IgM (light chain) mouse monoclonal antibody, clone 7A9

Reactivities Human

Human IgD mouse monoclonal antibody, clone IADB6, Purified

Applications ELISA.
Reactivities Human

Human IgD (Delta chain specific) mouse monoclonal antibody, clone IADB6, AP

Applications ELISA: 1/500-1/1,000.
Reactivities Human
Conjugation AP

Human IgD (Delta chain specific) mouse monoclonal antibody, clone IADB6, FITC

Applications Indirect Immunofluorescent staining of IgD+ human B lymphocytes: < / = 1 µg/10e6 cells.
Reactivities Human
Conjugation FITC

Human IgD (Delta chain specific) mouse monoclonal antibody, clone IADB6, HRP

Applications ELISA: 1/1,000-1/2,000.
Reactivities Human
Conjugation HRP

Human IgD (Delta chain specific) mouse monoclonal antibody, clone IADB6, PE

Applications Indirect Immunofluorescent staining of IgD+ human B lymphocytes: < / = 0.2 µg/10e6 cells.
Reactivities Human
Conjugation PE

Human IgE (Epsilon chain specific) mouse monoclonal antibody, clone HP6029, Biotin

Applications ELISA: 1/5,000-1/10,000
Reactivities Human
Conjugation Biotin

Human IgD mouse monoclonal antibody, clone IA6-2, Purified

Applications Flow cytometry.
Reactivities Human

Human IgD mouse monoclonal antibody, clone IA6-2, PE

Applications Flow cytometry analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivities Human
Conjugation PE

Goat IgG (Fc specific) mouse monoclonal antibody, clone SB115d, HRP

Applications ELISA: 1/10,000-1/20,000.
Reactivities Goat
Conjugation HRP

Rat IgG2c (heavy chain) mouse monoclonal antibody, clone MARG2c-5, FITC

Applications Flow cytometry: 5 µg/ml; use 10 µl of diluted antibody to label 10e6 cells in 100µl.
Reactivities Rat
Conjugation FITC

Rat IgG2c (heavy chain) mouse monoclonal antibody, clone MARG2c-5, Purified

Applications ELISA: 5 µg/ml as Coating antibody.
This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 as detection reagent and purified Rat IgG2c (Cat.No SA024).
Flow Cytometry: Use 10 µl of 1/200-1/1000 diluted antibody to label 106 cells in 100 µl.
Reactivities Rat