Secondary Antibodies

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Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-7, FITC

Applications Flow Cytometry: Use 10 µl of 2.5 µg/ml diluted antibody to label 106 cells in 100 µl.
Reactivities Mouse
Conjugation FITC

Mouse IgG (heavy chain) rat monoclonal antibody, clone LO-MG-7, HRP, Purified

Applications Immunoassay.
ELISA: good binding on plastic.
Immunohistochemistry on Frozen Sections. 
Reactivities Mouse
Conjugation HRP

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-7, Purified

Applications ELISA: 5µg/ml as coating antibody.
Reactivities Mouse

Mouse IgG1 (heavy chain) rat monoclonal antibody, clone LO-MG1-2, FITC

Applications Flow Cytometry: Use 10 µl of 5 µg/ml diluted antibody to label 106 cells in 100 µl.
Reactivities Mouse
Conjugation FITC

Rabbit Ig (IgG + IgM) rat monoclonal antibody, clone SB87a, Purified

Applications ELISA: 1/4,000-1/8,000.
Flow Cytometry.
Reactivities Rabbit

Mouse IgG1 (heavy chain) rat monoclonal antibody, clone LO-MG1-2, HRP

Applications ELISA: 500 ng/ml. 
This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with SM1491P  as the capture reagent.
Reactivities Mouse
Conjugation HRP

Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, Purified

Applications Flow Cytometry: Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells .
ELISA
FLISA
Immunohistochemistry (frozen sections).
Reactivities Mouse

Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells.
Reactivities Mouse
Conjugation PE

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, PE

Applications Flow Cytometry:  ≤ 0.2 µg/106 cells.
Identification and enumeration of IgG2a+.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+.
ELISA/FLISA.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation PE

Mouse IgA (alpha chain specific) rat monoclonal antibody, clone 11-44-2, Purified

Applications ELISA.
Flow Cytometry: Identification and enumeration of IgA+.
Immunofluorescence Microscopy: Identification and enumeration of IgA+.
Reactivities Mouse

Mouse IgG (heavy chain) rat monoclonal antibody, clone LO-MG-7, Biotin, Purified

Applications Immunoassays.
ELISA: good binding on plastic
Immunohistochemistry on paraffin sections. 
Indirect Immunofluorescence
on human peropheral blood cells.
Reactivities Mouse
Conjugation Biotin

Mouse IgG (heavy chain) rat monoclonal antibody, clone LO-MG-7, Purified

Applications ELISA (good binding on plastic) 
LO-MG-7 purified can be used at 0.6 µg/ml to obtain an OD of more than 1 on Mouse IgG2a en IgG2b.
Immunoassay: Useful as Secondary antibody.
Immunohistochemistry on Frozen Sections.
Reactivities Mouse

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-9, HRP

Applications ELISA (250 ng/ml).
Reactivities Mouse
Conjugation HRP

Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, Biotin

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells .
ELISA: 1/5000-1/10000.
Enzyme-Linked-Immunosorbent-Assay.
Reactivities Mouse
Conjugation Biotin

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone SB73a, Purified

Applications Flow Cytometry: < / 1 µg/10e6 cells
Identification and enumeration of IgM+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells .
ELISA: 1 µg/ml.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, Biotin

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells .
ELISA.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation Biotin

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, Purified

Applications Flow Cytometry: Identification and enumeration of IgM+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells.
ELISA.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, Biotin

Applications Flow Cytometry: Identification and enumeration of IgG2a+.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+.
ELISA.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation Biotin

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, FITC

Applications FLISA: 1/200-1/400. 
Flow Cytometry: ≤ 1 µg/106 cells.
Identification and enumeration of IgG2a+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation FITC

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, Purified

Applications Flow Cytometry: Identification and enumeration of IgG2a+.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+.
ELISA.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, PE

Applications Flow Cytometry:  ≤ 0.2 µg/106 cells.
Identification and enumeration of IgG2a+.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+.
ELISA/FLISA.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation PE

Mouse IgA (alpha chain specific) rat monoclonal antibody, clone 11-44-2, Biotin

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgA+.
Immunofluorescence Microscopy: Identification and enumeration of IgA+.
ELISA.
Reactivities Mouse
Conjugation Biotin

Mouse IgG (heavy chain) rat monoclonal antibody, clone LO-MG-7, FITC, Purified

Applications Immunoassay.
ELISA: good binding on plastic.
Immunohistochemistry on frozen sections.
Indirect Immunofluorescence.
Reactivities Mouse
Conjugation FITC

Mouse IgA (heavy chain) rat monoclonal antibody, clone LO-MA-7, Purified

Applications Immunoassay.
ELISA: good binding on plastic.
Immunohistochemistry on paraffin sections. 
Reactivities Mouse

Rabbit IgG (heavy chain) rat monoclonal antibody, clone RG-1, Aff - Purified

Applications ELISA.
Immunofluorescence.
Reactivities Rabbit

Mouse IgM (heavy chain) rat monoclonal antibody, clone LO-MM-9, FITC

Applications Flow Cytometry: 5 µg/ml; use 5 µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivities Mouse
Conjugation FITC

Mouse IgD (Delta heavy chain) rat monoclonal antibody, clone 11-26, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells. (See Ref.1-5)
Immunofluorescence.
Reactivities Mouse
Conjugation FITC

Mouse IgD (Delta heavy chain) rat monoclonal antibody, clone 11-26, Low Endotoxin

Applications Flow Cytometry (See Ref.1-4).
Functional Assays.
Reactivities Mouse

Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells.
Reactivities Mouse
Conjugation FITC

Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, Low Endotoxin

Applications Functional Assays.
Reactivities Mouse

Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells.
Reactivities Mouse
Conjugation PE

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, APC

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation APC

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgM+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation FITC

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, PE-Cy7

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation PE-Cy7

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation PE

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation PE

Mouse IgM (Mu chain specific), F(ab)2 Fragment rat monoclonal antibody, clone SB73a, Purified

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgM+.
Immunofluorescence Microscopy: Identification and enumeration of IgM+.
ELISA: < / = 1 µg/ml.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse

Mouse IgA (alpha chain specific) rat monoclonal antibody, clone 11-44-2, FITC

Applications FLISA: 1/200-1/400.
Flow Cytometry:
 ≤ 1 µg/106 cells.
Identification and enumeration of IgA+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgA+ cells.
Reactivities Mouse
Conjugation FITC

Mouse IgA (alpha chain specific) rat monoclonal antibody, clone 11-44-2, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgA+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgA+ cells.
Reactivities Mouse
Conjugation PE

Mouse IgA (alpha chain specific) rat monoclonal antibody, clone 11-44-2, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgA+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgA+ cells.
Reactivities Mouse
Conjugation PE

Rabbit Ig (IgG + IgM) rat monoclonal antibody, clone SB87a, Biotin

Applications ELISA: 1/5,000-1/20,000.
Flow Cytometry.
Reactivities Rabbit
Conjugation Biotin

Rabbit Ig (IgG + IgM) rat monoclonal antibody, clone SB87a, HRP

Applications ELISA: 1/4,000-1/8,000.
Reactivities Rabbit
Conjugation HRP

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-2, Biotin, Aff - Purified

Reactivities Mouse
Conjugation Biotin

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-2, HRP, Aff - Purified

Reactivities Mouse
Conjugation HRP

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-2, Aff - Purified

Applications Immunoassay.
Immunohistochemistry on Frozen Sections. 
The specificity of monoclonal antibody anti-IgG subclasses is determined in a range of optimal concentrations. Increasing of the first or second antibody at a high level can induce cross-reactions with other IgG subclasses.
Reactivities Mouse

Mouse IgA (heavy chain) rat monoclonal antibody, clone LO-MA-7, Biotin, Purified

Applications Immunoassay.
ELISA: good binding on plastic.
Immunohistochemistry on frozen sections. 
Reactivities Mouse
Conjugation Biotin

Mouse IgA (heavy chain) rat monoclonal antibody, clone LO-MA-7, HRP, Purified

Applications Immunoassay.
ELISA: good binding on plastic.
Immunohistochemistry on frozen sections. 
Reactivities Mouse
Conjugation HRP

Human IgM rat monoclonal antibody, clone HM-7, Aff - Purified

Applications ELISA.
Reactivities Human

Mouse IgG1 (heavy chain) rat monoclonal antibody, clone LO-MG1-2, Purified

Applications Useful as second antibody in immunoassays, such as ELISA's (good binding on plastic), Immunohistochemistry on Frozen Sections and for Affinity Chromatography of murine IgG1.
Reactivities Mouse

Mouse IgG3 (heavy chain) rat monoclonal antibody, clone LO-MG3-7, FITC, Purified

Applications

Useful as second antibody in Immunoassays, such as ELISA's (good binding on plastic) and Immunohistochemistry on Frozen Sections.
The antibody can be used at 5 μg/ml.

Reactivities Mouse
Conjugation FITC