Mouse IgG (H+L chain) sheep polyclonal antibody, Biotin
| Applications | Suiatble for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western Blot: 1/1,000-1/5,000. Immunohistochemistry: 1/1,000-1/2,000. |
| Conjugation | Biotin |
| Immunogen | Mouse IgG whole molecule. |
Guinea Pig IgG (Fc specific) sheep polyclonal antibody, Biotin
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. In immunocytochemical and immunohistochemical staining of IgG at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of guinea pig origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in guinea pig serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000. |
| Reactivities | Guinea Pig |
| Conjugation | Biotin |
| Immunogen | Purified normal IgG isolated from pooled guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Rabbit IgG (H+L chain) sheep polyclonal antibody, Biotin
| Applications | ELISA: 1/25600 - 1/51200. Western Blot: 1/20000. Immunohistochemistry on frozen sections: 1/400 - 1/800. Immunohistochemistry on paraffin sections: 1/100 - 1/200. |
| Reactivities | Rabbit |
| Conjugation | Biotin |
| Immunogen | Rabbit IgG from pooled sera |
Rabbit IgG (H+L chain) sheep polyclonal antibody, Biotin
| Applications | Immunoblotting, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. ELISA: 1:300.000. Western blot and Immunohistochemistry: user optimized. |
| Conjugation | Biotin |
| Immunogen | Rabbit IgG whole molecule |
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