Secondary Antibodies

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Mouse IgG (H+L chain) sheep polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse

Human IgG3 sheep polyclonal antibody, HRP

Applications ELISA.
Immunohistochemistry on Frozen Sections: 1/100-1/200.
Reactivities Human
Conjugation HRP

Mouse IgG (H+L chain) sheep polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/10,000-1/200,000
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Mouse
Conjugation HRP

Bovine IgG1 sheep polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Reactivities Bovine
Conjugation AP

Human IgA (Fc specific) sheep polyclonal antibody, TRITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgA. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/10-1/40
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation TRITC

Human IgG (Fc specific) sheep polyclonal antibody, TRITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgG. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/10-1/40
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation TRITC

Guinea Pig IgG (Fc specific) sheep polyclonal antibody, Biotin

Applications ELISA.
Dot blot. 
Immunoblotting.

Immunocytochemistry.
Immunohistochemistry on Paraffin Sections.
In immunocytochemical and immunohistochemical staining of IgG at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of guinea pig origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in guinea pig serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000.
Reactivities Guinea Pig
Conjugation Biotin

Guinea Pig IgG (Fc specific) sheep polyclonal antibody, HRP

Applications ELISA.
Dot blot. 
Immunoblotting.

Immunocytochemistry.
Immunohistochemistry on Paraffin Sections.
Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of guinea pig origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in guinea pig serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000.
Reactivities Guinea Pig
Conjugation HRP

Bovine IgG1 sheep polyclonal antibody, Aff - Purified

Applications ELISA.
Immunodiffusion.
Reactivities Bovine

Rabbit IgG sheep polyclonal antibody, Purified

Applications ELISA.
Western Blot: 1/1000.
Immunohistochemistry on Frozen Sections.
Reactivities Rabbit

Guinea Pig IgG (Fc specific) sheep polyclonal antibody, FITC

Applications ELISA.
(In)direct immunofluorescence.

Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Can be  used in direct staining of cytoplasmic IgG in fixed guinea pig cells and tissue substrates; to identify circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of guinea pig in the middle layer of the test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80.
Reactivities Guinea Pig
Conjugation FITC

Bovine IgG2 sheep polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Reactivities Bovine
Conjugation AP

Human IgG1 sheep polyclonal antibody, Purified

Applications ELISA: 1/4000-1/8000
Western Blot: 1/4000-1/8000
Reactivities Human

Bovine IgG sheep polyclonal antibody, AP

Applications

ELISA: 1/5000-1/50000.
Western Blot: 1/5000-1/50000.

Reactivities Bovine
Conjugation AP

Bovine IgG sheep polyclonal antibody, HRP

Applications ELISA: 1/10,000-1/100,000. 
Western Blot.
 
Reactivities Bovine
Conjugation HRP

Bovine IgG sheep polyclonal antibody, Purified

Applications ELISA: 1/100-1/10,000.
Immunodiffusion.
Reactivities Bovine

Bovine IgM sheep polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000. 
Western Blot: 1/5000-1/50000.
Reactivities Bovine
Conjugation AP

Bovine IgM sheep polyclonal antibody, Aff - Purified

Applications ELISA: 1/100-1/1000.
Immunodiffusion.
Reactivities Bovine

Bovine IgG2 sheep polyclonal antibody, Aff - Purified

Applications ELISA: 1/100 - 1/10000.
Immunodiffusion.
Reactivities Bovine

Rabbit IgG (H+L chain) sheep polyclonal antibody, Biotin

Applications ELISA: 1/25600 - 1/51200.
Western Blot: 1/20000.
Immunohistochemistry on frozen sections: 1/400 - 1/800.
Immunohistochemistry on paraffin sections: 1/100 - 1/200.
Reactivities Rabbit
Conjugation Biotin

Bovine IgM sheep polyclonal antibody, HRP

Applications ELISA: 1/10,000 - 1/100,000.
Reactivities Bovine
Conjugation HRP

Bovine IgG1 sheep polyclonal antibody, HRP

Applications ELISA: 1/10000-1/100000.
Reactivities Bovine
Conjugation HRP

Bovine IgG2 sheep polyclonal antibody, HRP

Applications ELISA: 1:10,000-1:100,000.
Reactivities Bovine
Conjugation HRP