Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, HRP
| Applications | Sandwich ELISA: 1/120,000 dilution of antibody was found to generate an O.D of 1.0 in a 15 minute reaction with Tetramethyl Benzidine as the substrate. Western blot (1/5000). Immunoelectrophoresis. |
| Reactivities | Chicken |
| Conjugation | HRP |
| Immunogen | Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected. |
Goat IgG (H+L chain) chicken polyclonal antibody, HRP
| Applications | ELISA: a 1/75,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate. Western Blot (1/5000). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in Agarose gels and ELISA analysis. |
| Reactivities | Goat |
| Conjugation | HRP |
| Immunogen | Purified, non-denatured Goat IgG (containing both heavy and light chains) emulsified in Freund's adjuvant. Production: After multiple injections, eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-goat IgG's were affinity-purified over a column conjugated with purified Goat IgG (containing both heavy and light chains) and then conjugated with Horseradish Peroxidase. |
Human IgG (H+L chain) chicken polyclonal antibody, HRP
| Applications | ELISA: a 1/300,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethyl-benzidine as substrate. Western Blot (1/5000). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and ELISA analysis. |
| Reactivities | Human |
| Conjugation | HRP |
| Immunogen | Purified, non-denatured Human IgG (containing both heavy and light chains). Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Human IgG's were affinity-purified over a column conjugated with purified Human IgG (containing both heavy and light chains) and then conjugated with Horseradish Peroxidase. |
Mouse IgG (H+L chain) chicken polyclonal antibody, HRP
| Applications | ELISA: a 1/70,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate. Western Blot: 1/200 -1/5000. Immunocytochemistry. Immunohistochemistry: 1/250 - 1/5000. Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose gels and ELISA analysis. |
| Reactivities | Mouse |
| Conjugation | HRP |
| Immunogen | Purified, non-denatured Mouse IgG (containing both heavy and light chains). Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Mouse IgG's were affinity-purified over a column conjugated with purified Mouse IgG (containing both heavy and light chains) and conjugated to Horseradish peroxidase. |
Rabbit IgG (H+L chain) chicken polyclonal antibody, HRP
| Applications | ELISA: a 1/20,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate. Western Blot (1/5000). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose gels and ELISA analysis. |
| Reactivities | Rabbit |
| Conjugation | HRP |
| Immunogen | Purified, non-denatured Rabbit IgG (containing both heavy and light chains). Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Rabbit IgG's were affinity-purified over a column conjugated with purified Rabbit IgG (containing both heavy and light chains) and then conjugated to Horseradish Peroxidase. |
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