Secondary Antibodies

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Rat IgD (heavy chain) mouse monoclonal antibody, clone MARD-3, Purified

Applications Immunohistochemistry on Frozen Sections.
ELISA: This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 (Cat.-No AM05512HR-N) as detection reagent.
Reactivities Rat

Human IgG (F(ab)2 specific) mouse monoclonal antibody, clone 4A11, Purified

Applications ELISA.
Western blotting.
Flow Cytometry.

Has been described for previous lots to work in Immunofluorescence.
Reactivities Human

Human IgM (Fc-region) mouse monoclonal antibody, clone CH2, Purified

Applications Western blot.
Flow cytometry: 1µg/ml.
Immunocytochemistry.
ELISA.
Reactivities Human

Canine IgM goat polyclonal antibody, Aff - Purified

Applications ELISA: 1/100-1/1000.
Flow Cytometry.
Immunodiffusion.
Immunohistochemistry on Frozen Sections.
Reactivities Canine

Human IgE mouse monoclonal antibody, clone 4H10, Purified

Applications Flow Cytometry: 4 µg/ml.
ELISA.
Reactivities Human

Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, Biotin

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells .
ELISA: 1/5000-1/10000.
Enzyme-Linked-Immunosorbent-Assay.
Reactivities Mouse
Conjugation Biotin

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone SB73a, Purified

Applications Flow Cytometry: < / 1 µg/10e6 cells
Identification and enumeration of IgM+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells .
ELISA: 1 µg/ml.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, Biotin

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells .
ELISA.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation Biotin

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, Purified

Applications Flow Cytometry: Identification and enumeration of IgM+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells.
ELISA.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, Biotin

Applications Flow Cytometry: Identification and enumeration of IgG2a+.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+.
ELISA.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation Biotin

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, FITC

Applications FLISA: 1/200-1/400. 
Flow Cytometry: ≤ 1 µg/106 cells.
Identification and enumeration of IgG2a+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation FITC

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, Purified

Applications Flow Cytometry: Identification and enumeration of IgG2a+.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+.
ELISA.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, PE

Applications Flow Cytometry:  ≤ 0.2 µg/106 cells.
Identification and enumeration of IgG2a+.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+.
ELISA/FLISA.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation PE

Mouse IgA (alpha chain specific) rat monoclonal antibody, clone 11-44-2, Biotin

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgA+.
Immunofluorescence Microscopy: Identification and enumeration of IgA+.
ELISA.
Reactivities Mouse
Conjugation Biotin

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 7E7, Biotin

Applications Flow Cytometry: ≤ 1 µg/106 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
ELISA: 1/5,000-1/20,000.
Reactivities Rat
Conjugation Biotin

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 7E7, Purified

Applications Flow Cytometry.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
ELISA.
Second step reagent for rat IgG1 Monoclonal Antibodies.
Reactivities Rat

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, Biotin

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
ELISA: Can be used as Capture antibody.
Reactivities Rat
Conjugation Biotin

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, FITC

Applications FLISA: 1/200-1/400.
Flow Cytometry:
  1 ≤ µg/106 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
Reactivities Rat
Conjugation FITC

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, Purified

Applications Flow Cytometry.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
ELISA: Can be used as Capture antibody.
Second step reagent for rat IgG1 Monoclonal Antibodies.
Reactivities Rat

Rat IgG2a (gamma 2a chain specific) mouse monoclonal antibody, clone 2A 8F4, Purified

Applications Flow Cytometry.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
ELISA.
Second step reagent for rat IgG2a Monoclonal Antibodies.
Reactivities Rat

Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, Purified

Applications Flow Cytometry.
Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells.
ELISA.
Second step reagent for rat IgG2c Monoclonal Antibodies.
Reactivities Rat

Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, PE

Applications Flow Cytometry: FITC and BIOT conjugates ≤1 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells.
ELISA.
Reactivities Rat
Conjugation PE

Rat IgM (Mu chain specific) mouse monoclonal antibody, Biotin

Applications Flow Cytometry (< / = 1.0 µg/10e6 cells).
Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells.
ELISA.
Second step reagent for rat IgM Monoclonal Antibodies.
Surface IgM Detection.
B Cell Stimulation.
Reactivities Rat
Conjugation Biotin

Rat IgM (Mu chain specific) mouse monoclonal antibody, clone M 2A1, Purified

Applications Flow Cytometry and Immunofluorescence Microscopy:
Identification and enumeration of IgM+ cells.
ELISA.
Second step reagent for rat IgM Monoclonal Antibodies.
Surface IgM Detection.
B Cell Stimulation.

Rabbit IgG mouse monoclonal antibody, clone 2A9, Biotin

Applications ELISA: 1/5,000-1/20,000.
Flow Cytometry.
Reactivities Rabbit
Conjugation Biotin

Chicken IgM (Mu heavy chain specific) mouse monoclonal antibody, clone M-4, Purified

Applications Flow Cytometry: ≤ 1 µg/106 cells.
Indirect Immunofluorescence staining. (Ref.1)
In ovo suppression of Chicken B lymphocytes.
In vitro activation of Chicken peripheral B lymphocytes.
Reactivities Chicken

Chicken IgA (alpha chain specific) mouse monoclonal antibody, clone A-1, Biotin

Applications ELISA.
Flow Cytometry: < / = 1 µg/10e6 cells.
Immunofluorescence.
Conjugation Biotin

IGHG1 (heavy chain) mouse monoclonal antibody, clone ICO-97, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT.
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human

IGHG1 (heavy chain) mouse monoclonal antibody, clone ICO-97, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT.
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human

Rat IgG1 goat polyclonal antibody, Purified

Applications ELISA: 1-10 µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Rat

Rat IgG2b rabbit polyclonal antibody, Purified

Applications ELISA: 1µg/ml - 10µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Rat

Rat IgG2c rabbit polyclonal antibody, Purified

Applications ELISA: 1µg/ml - 10µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Human, Rat

Rat IgM goat polyclonal antibody, Purified

Applications ELISA 1ug/ml - 10ug/ml, Western Blotting, Flow Cytometry
Reactivities Rat

Mouse IgG (adsorbed) goat polyclonal antibody, Purified

Applications ELISA: 1µg/ml - 10µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Mouse

Mouse IgG2a goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/10.
Immunofluorescence: 1/10.
Reactivities Mouse
Conjugation FITC

Mouse IgG2b goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 106 cells in 100 µl.
Immunocytochemistry: Neat-1/10. 
Immunohistochemistry on Frozen Sections.
Reactivities Mouse
Conjugation FITC

Mouse IgA goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10e6 cells in 100 µl.
Immunofluorescence: neat-1/10.
Immunohistochemistry on Frozen Sections.
Reactivities Mouse, Rat
Conjugation FITC

Human IgD (heavy chain) goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 106 cells in 100 µl.
Immunocytochemistry: Neat-1/10.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation FITC

Human IgD (F(ab)2 Fragment) goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 10e6 cells in 100 µl.
Immunofluorescence: Neat-1/10.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation FITC

Mouse IgG3 goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 106 cells in 100 µl.
Immunocytochemistry: Neat-1/10.
Immunohistochemistry on Frozen Sections.
Reactivities Mouse
Conjugation FITC

Mouse IgA (alpha chain specific) goat polyclonal antibody, Texas Red

Applications FLISA: 1/100-1/400.
Flow Cytometry.
Immunohistochemistry.
Reactivities Mouse
Conjugation Texas Red

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Texas Red

Applications

FLISA: 1/100–1/400.
Flow Cytometry.
Immunohistochemistry.

Reactivities Mouse
Conjugation Texas Red

Human IgE mouse monoclonal antibody, clone I27/mAb27

Applications FACS surface, Western Blot, Basophil degranulation, Study of IgE binding to CD23 (Fc epsilon R2). 
Usage recommendation
:
*This monoclonal antibody may be used between 1-10 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
Reactivities Human

Goat IgG (H+L chain), adsorbed rabbit polyclonal antibody, TRITC

Applications Suitable for ELISA, Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA:  1/10,000 - 1/50,000.
IF Microscopy:  1/1000 - 1/5000.
Conjugation TRITC

Rabbit IgG (H+L chain) (Fab Fragment) goat polyclonal antibody, FITC

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
Flow cytometry 1:2,000 - 1:10,000
Immunofluorescence 1:1,000 - 1:5,000
FLISA:1:10,000-1:50,000.
Conjugation FITC

Mouse IgM (heavy chain) rat monoclonal antibody, clone LO-MM-9, FITC

Applications Flow Cytometry: 5 µg/ml; use 5 µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivities Mouse
Conjugation FITC

Chicken IgG / Chicken IgY (heavy chain) mouse monoclonal antibody, clone 16C7, Purified

Applications Flow Cytometry.
ELISA.
Western Blot under non-reducing conditions.
Reactivities Duck

Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, Biotin

Applications ELISA: 1/1000 - 1/2000.
Flow Cytometry: 1/50 - 1/100; Use 50µl of the suggested working dilution to label 10e6 cells in 100µl.
Immunohistochemistry on frozen and paraffin embedded sections: 1/50 - 1/200.
Reactivities Mouse
Conjugation Biotin