Mouse IgG (H+L chain) sheep polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/100,000. Western blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000. |
| Reactivities | Mouse |
Human IgA (Fc specific) sheep polyclonal antibody, TRITC
| Applications | Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgA. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected. Recommended Dilutions: 1/10-1/40 This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. |
| Reactivities | Human |
| Conjugation | TRITC |
Human IgG (Fc specific) sheep polyclonal antibody, TRITC
| Applications | Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgG. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected. Recommended Dilutions: 1/10-1/40 This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. |
| Reactivities | Human |
| Conjugation | TRITC |
Guinea Pig IgM (Fc specific) sheep polyclonal antibody, Serum
| Applications | Immunoprecipitation. Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in guinea pig serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of guinea pig IgM from serum or plasma. Directions for Use: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody titre: Precipitin titre not less than 1/32 when tested against normal guinea pig serum in agar block titration. |
| Reactivities | Guinea Pig |
Bovine IgG1 sheep polyclonal antibody, Aff - Purified
| Applications | ELISA. Immunodiffusion. |
| Reactivities | Bovine |
Human IgA (secretory) sheep polyclonal antibody, Serum
| Applications | Precipitation. Can be used in precipitating as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgA in Human serum, milk or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of Human IgA from serum or exocrine secretions. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms as secretory IgA. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. This does not exclude such use if proper controls are include. Recommended Dilutions: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion: Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody Titre: Precipitin titre not less than 1/64 when tested against pooled normal human serum in agar-block immunodiffusion titration. |
| Reactivities | Human |
Bovine IgG2 (subclass specific) sheep polyclonal antibody, Serum
| Applications | This antibody can be used in precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgG2 in Bovine serum and other body fluids or to determine its concentration. Recommneded Dilutions: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody titre: Precipitin titre not less than 1/32 when tested against normal goat serum in agar block titration. |
| Reactivities | Bovine |
Canine IgG (Fc specific) sheep polyclonal antibody, Serum
| Applications | Immunoprecipitation. Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG in dog serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of dog IgG from serum or plasma. The antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive techniques. For such uses labelled and unlabelled cytochemical grade reagents raised in goats meeting the necessary additional specificity and performance requirements are available. Directions for Use: Immunoelectrophoresis: Use 2 μl serum or equivalent against 120 μl antiserum. Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells. Precipitin titre: 1/16 when tested against pooled normal dog serum in agar-block immunodiffusion titration. |
| Reactivities | Canine |
Guinea Pig IgA (Fc specific) sheep polyclonal antibody, Serum
| Applications | Immunoprecipitation. Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in guinea pig serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of guinea pig IgA from serum or plasma. This antiserum is not intended for use in non-precipitating antibodybinding or other highly sensitive assays. This does not exclude such use if [roper controls are included. Directions for Use: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody titre: Precipitin titre not less than 1/40 when tested against normal guinea pig serum in agar block titration. |
| Reactivities | Guinea Pig |
Guinea Pig IgG1 (subclass specific) sheep polyclonal antibody, Serum
| Applications | Immunoprecipitation. Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG1 in guinea pig serum and other body fluids or to determine its concentration. Directions for Use: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody titre: Precipitin titre not less than 1/16 when tested against normal guinea pig serum in agar block titration. |
| Reactivities | Guinea Pig |
Bovine IgG sheep polyclonal antibody, Purified
| Applications | ELISA: 1/100-1/10,000. Immunodiffusion. |
| Reactivities | Bovine |
Bovine IgM sheep polyclonal antibody, Aff - Purified
| Applications | ELISA: 1/100-1/1000. Immunodiffusion. |
| Reactivities | Bovine |
Bovine IgG2 sheep polyclonal antibody, Aff - Purified
| Applications | ELISA: 1/100 - 1/10000. Immunodiffusion. |
| Reactivities | Bovine |
Germany
Japan
United Kingdom
China