Secondary Antibodies

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Monkey IgG (Fc specific) goat polyclonal antibody, FITC

Applications Can be used:
• In direct staining of cytoplasmic IgG in fixed Monkey cells and tissue substrates.
• To identify circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases.
• To identify a specific antigen or immune complex using a reference antibody of Monkey in the middle layer of the test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilution: 1/20-1/80.
Reactivities Monkey
Conjugation FITC

Mouse IgG (H+L chain) sheep polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse

Mouse IgG (Fc specific) goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000. 
Western blot: 1/2,000-1/10,000. 
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse

Mouse IgD (Fc specific) goat polyclonal antibody, Biotin

Applications In Immunocytochemical and Immunohistochemical staining of IgD at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgD antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgD isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgD in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/200-1/1000.
Reactivities Mouse
Conjugation Biotin

Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining to identify and measure immunoglobulins, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rabbit origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and Cytochemical: 1/100 - 1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/5000 - 1/20000.
Reactivities Rabbit
Conjugation Biotin

Monkey IgG (Fab specific) rabbit polyclonal antibody, FITC

Applications Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1:20 and 1:80.
Reactivities Monkey
Conjugation FITC

Mouse IgE (Fc specific) goat polyclonal antibody, HRP

Applications In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgE antibodies in mouse serum or other body fluids; in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
depending on the method used.
Reactivities Mouse
Conjugation HRP

Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, HRP

Applications ELISA (to identify and measure a specific IgG in rabbit serum or other body fluids).
Immunocytochemistry.
Immunohistochemistry.
Useful in electron microscopy, since the complex between the conjugated antibody and the antigen has electron-dense properties.
Dot blot.
Western blot.
General Recommended Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Working dilutions should be prepared by adding sterile PBS, pH 7.2, and should not be refrozen.
Reactivities Rabbit
Conjugation HRP

Human IgA (Secretory component) goat polyclonal antibody, Biotin

Applications This antibody can be used In Immunocytochemical and Immunohistochemical staining for the detection of free SC and secretory IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. In non-isotopic assay methodology (e.g. ELISA) to identify and measure SC or secretory IgA in Human body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted and the optimum working dilution should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/8,000.
Reactivities Human
Conjugation Biotin

Human IgA (Secretory component) goat polyclonal antibody, HRP

Applications In enzyme-immunocytochemical and immunohistochemical staining of free or bound secretory component at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA, Western blotting) in human milk or other secretions. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/6000.
Reactivities Human
Conjugation HRP

Monkey IgG (Fab specific) rabbit polyclonal antibody, HRP

Applications Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells.
Combinations of isotype-specific reagents should be used instead for this purpose.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommneded Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/5000-1/10000.
Reactivities Monkey
Conjugation HRP

Duck IgM (Fc specific) goat polyclonal antibody, HRP

Applications Suitable for use in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of Duck origin known to be of the IgM isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgM in duck serum or other body fluids.
Recommended Dilutions:
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/3,000.
Immunohistochemistry and Cytochemistry: 1/50-1/250.
Note: This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Duck
Conjugation HRP

Human IgE (Fc specific) goat polyclonal antibody, FITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to identify and measure IgE in Human serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80, depending on the method used.
Reactivities Human
Conjugation FITC

Human IgE (Fc specific) goat polyclonal antibody, HRP

Applications Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To demonstrate circulating IgE antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using an reference antibody of Human origin known to be of the IgE isotype in the middle layer of the indirect test procedure.
In non-isotopic assay methodology (e.g. ELISA) to measure IgE in Human serum or other body fluids.
Recommneded Dilutions:
Histochemistry and Cytochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/25,000.
Reactivities Human
Conjugation HRP

Rat IgG2b (subclass specific) goat polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgG2b at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG2b antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rat origin known to be of the IgG2b isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG2b in rat serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/10000.
Reactivities Rat
Conjugation Biotin

Monkey IgG (Fc specific) goat polyclonal antibody, HRP

Applications Dot blot.
Immunoblotting. 
ELISA.
Immunocytochemistry.
Immunohistochemistry on Paraffin Sections.
This antibody can be used:
In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using a reference antibody of monkey origin known to be of the IgG isotype in the middle layer of the indirect test procedure
In non-isotopic assay methodology (e.g. ELISA) to measure IgG in monkey serum or other body fluids.
This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histo- and Cytochemistry: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/5000-1/20000.
Reactivities Monkey
Conjugation HRP

Monkey IgG (Fab specific) rabbit polyclonal antibody, Azide Free

Applications Can be used for indirect staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa and lambda type. In general this kind of products is not recommended as direct or indirect screening reagents for immunoglobulin isotypes on the surface of membranes of vital lymphoid cells. The presence of activity to the common Fab subunit may result in the staining of Ig bound to Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemistry Use: 1/50-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Reactivities Monkey

Monkey IgG (Fab specific) rabbit polyclonal antibody, Biotin

Applications In immunocytochemical and immunohistochemical staining to identify and measure free or Ig bound subunits of both kappa and lambda type at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. In general this kind of products is not recommended as direct or indirect screening reagents for immunoglobulin isotypes on the surface of membranes of vital lymphoid cells. The presence of activity to the common Fab subunit may result in the staining of Ig bound to Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000.
Reactivities Monkey
Conjugation Biotin

Mouse IgG (Fc specific) goat polyclonal antibody, AP

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/2,000-1/100,000
Western blot: 1/500-1/2,500
Immunohistochemistry: 1/200-1/1,000.
Note: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code #NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1/500 to 1/5,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation AP

Chicken IgM (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of chicken origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in chicken serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Reactivities Chicken
Conjugation HRP

Duck IgM (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgM in duck serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of Duck IgM from serum or plasma.
Recommended Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl in a 3 mm diameter center well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Precipitin titre: 1/16 when tested against pooled normal duck serum in agar-block immunodiffusion titration.
Reactivities Duck

Guinea Pig IgG2 (subclass specific) goat polyclonal antibody, Biotin

Applications In immunocytochemical and immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG2 antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of guinea pig origin known to be of the IgG2 isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG2 in guinea pig serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
Histochemical and Cytochemical: 1/50 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/200 - 1/2000.
Reactivities Guinea Pig
Conjugation Biotin

Guinea Pig IgG2 (subclass specific) goat polyclonal antibody, HRP

Applications This antibody can be used in Enzyme Immunocytochemical and Immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To demonstrate circulating IgG2 antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of guinea pig origin known to be of the IgG2 isotype in the middle layer of the indirect test procedure In non-isotopic assay methodology (e.g. ELISA) to measure IgG2 in guinea pig serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions:
Histochemical and Cytochemical Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,0000.
Reactivities Guinea Pig
Conjugation HRP

Guinea Pig IgM (Fc specific) goat polyclonal antibody, Biotin

Applications In immunocytochemical and immunohistochemical use for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in guinea pig serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
Histochemical and Cytochemical: 1/100 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000 - 1/5000.
Reactivities Guinea Pig
Conjugation Biotin

Guinea Pig IgM (Fc specific) goat polyclonal antibody, HRP

Applications Can be used for Enzyme-Immunocytochemical and immunohistochemical detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of Guinea Pig origin known to be of the IgM isotype in the middle layer of the indirect test procedure. In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Guinea Pig serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical: 1/100-1/250. 
ELISA and comparable non-precipitating antibody-binding assays: 1/400-1/4000.
Reactivities Guinea Pig
Conjugation HRP

Human IgA (Secretory component) goat polyclonal antibody, FITC

Applications Can be used as reagent for the direct detection of secretory component in human cells, tissues and body fluids in Immunofluorescence, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA).
This immunoconjugate is not pre-diluted and the optimum working dilution should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions: 1/20-1/80.
Reactivities Human
Conjugation FITC

Human IgD (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgD at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology.
In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgD in human serum or other body fluid.
As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000.
Performance testing. Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of human cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays.
Reactivities Human
Conjugation Biotin

Human IgD (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in Enzyme-immunocytochemical and Immunohistochemical staining for the detection of IgD at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgD antibodies in serodiagnostic microbiology and autoimmune diseases; in nonisotopic assay methodology (e.g. ELISA) to measure IgD in human serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and Cytochemical: 1/50 - 1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/10,000.
Reactivities Human
Conjugation HRP

Mouse IgD (Fc specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble Immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase Immunochemistry. When applied in any Cytochemical or Histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemical Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.
Reactivities Mouse

Mouse IgD (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgD in mouse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of mouse IgD from serum or plasma. This antiserum is not intended for use in non-precipitating antibodybinding or other highly sensitive assays. For such use labelled an unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity an performance requirements are available.
Antibody titre: Precipitin titre 1/32 when tested against monoclonal IgD containing mouse serum in agarblock immunodiffusion titration.
Reactivities Mouse

Mouse IgE (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgE in mouse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of mouse IgE from serum or plasma. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such use labelled an unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity an performance requirements are available.
Antibody titre: Precipitin titre 1/32 when tested against monoclonal IgE containing mouse serum in agarblock immunodiffusion titration.
Reactivities Mouse

Rat IgE (Fc specific) goat polyclonal antibody, Biotin

Applications This antibody can be used
In Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology.
In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in rat serum or other body fluid.
As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions:
Histochemistry and Cytochemistry: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000.
Reactivities Rat
Conjugation Biotin

Rat IgE (Fc specific) goat polyclonal antibody, HRP

Applications This antibody can be used
In Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology.
In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in rat serum or other body fluid.
As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions:
Histochemistry and Cytochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000.
Reactivities Rat
Conjugation HRP

Mouse IgE (Fc specific) rabbit polyclonal antibody, Azide Free

Applications Can be used as unlabelled secondary antibody for indirect detection of IgE in Mouse cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in Mouse serum or other body fluid. When applied in any Immunocytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.
Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays.
Reactivities Mouse

Mouse IgE (Fc specific) rabbit polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology. In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluid. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/3000.
Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays.
Reactivities Mouse
Conjugation Biotin

Mouse IgE (Fc specific) rabbit polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgE antibodies in mouse serum or other body fluids; in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and Cytochemical: 1/100 - 1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000.
Reactivities Mouse
Conjugation HRP

Rabbit IgM (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical use for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in rabbit serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and Cytochemical: 1/100 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000 - 1/10000.
Reactivities Rabbit
Conjugation Biotin

Rabbit IgM (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rabbit origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in rabbit serum or other body fluids.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/10000.
Reactivities Rabbit
Conjugation HRP

Rabbit IgA (Fc specific) goat polyclonal antibody, Biotin

Applications In immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rabbit origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in rabbit serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and Cytochemical: 1/50 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/100 - 1/1000.
Reactivities Rabbit
Conjugation Biotin

Rabbit IgA (Fc specific) goat polyclonal antibody, FITC

Applications In immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rabbit origin known to be of the IgA isotype in the middle layer of the indirect test procedure. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/20 - 1/80.
Reactivities Rabbit
Conjugation FITC

Rabbit IgA (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rabbit origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in rabbit serum or other body fluids. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and Cytochemical: 1/50 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000.
Reactivities Rabbit
Conjugation HRP

Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Azide Free

Applications The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.
Reactivities Rabbit

Rat IgG2b (subclass specific) goat polyclonal antibody, HRP

Applications Can be used in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgG2b at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to demonstrate circulating IgG2b antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of rat origin known to be of the IgG2b isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgG2b in Rat serum or other body fluids.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used.
Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/10000.
Reactivities Rat
Conjugation HRP

Rat IgG2ab (subclass specific) goat polyclonal antibody, HRP

Applications Can be used in Enzyme-immunocytochemical and Immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG2 antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rat origin known to be of the IgG2 isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG2 in rat serum or other body fluids.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and Cytochemical: 1/100 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000 - 1/10,000.
Reactivities Rat
Conjugation HRP

Mouse IgG3 (subclass specific) goat polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgG3 at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG3 antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgG3 isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG3 in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions
Histochemical and Cytochemical: 1/100 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1.000 - 1/5,000.
Reactivities Mouse
Conjugation Biotin

Mouse IgG3 (subclass specific) goat polyclonal antibody, FITC

Applications This antibody can be used to identify and measure IgG3, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of mouse origin in the middle layer of the indirect test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions: 1/20-1/80.
Reactivities Mouse
Conjugation FITC

Mouse IgG3 (subclass specific) goat polyclonal antibody, HRP

Applications This antibody can be used in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgG3 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates To demonstrate circulating IgG3 antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using a reference antibody of mouse origin known to be of the IgG3 isotype in the middle layer of the indirect test procedure In non-isotopic assay methodology (e.g. ELISA) to measure IgG3 in Mouse serum or other body fluids.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions:
Histochemical/Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/7,000 
depending on the method used. Working dilutions schould be stored at +4°C, not refrozen, and preferably used the same day.
Reactivities Mouse
Conjugation HRP

Bovine IgM (Fc specific) rabbit polyclonal antibody, Biotin

Applications Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using an reference antibody of bovine origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Bovine serum or other body fluids.
As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
Recommneded Dilutions:
Histochemistry and Cytochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/100-1/500.
Reactivities Bovine
Conjugation Biotin

Bovine IgM (Fc specific) rabbit polyclonal antibody, HRP

Applications Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using an reference antibody of bovine origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Bovine serum or other body fluids.
Recommneded Dilutions:
Histochemistry and Cytochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/2,000.
Reactivities Bovine
Conjugation HRP

Monkey IgA + IgG + IgM (H+L chain) goat polyclonal antibody, Biotin

Applications Can be used in immunocytochemical and immunohistochemical staining of immunoglobulins at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure immunoglobulins in monkey serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/2000 and 1/10000.
Reactivities Monkey
Conjugation Biotin