Secondary Antibodies

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Mouse IgM (Mu chain specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
The maximum amount of reagent required to stain 1 x 10e6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate.
Recommended dilutions:
Flow cytometry 1:100 - 1:250
IF microscopy 1:100 - 1:250
Conjugation PE

Rabbit IgG F(ab)2 Fragment (H+L chain) donkey polyclonal antibody, PE

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
Immunofluorescence: 1/100-1/200.
Flow Cytometry: 1/100-1/20. The maximum amount of reagent required to stain 1 x 10e6 cells is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Reactivities Rabbit
Conjugation PE

Rat IgG (H+L chain) F(ab')2 Fragment donkey polyclonal antibody, PE

Applications Suitable for Immunomicroscopy (1:100-1:200) and Flow Cytometry (1:100-1:200) or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Conjugation PE

Rat IgM (Mu chain specific) goat polyclonal antibody, PE

Applications Suitable for immunomicroscopy and flow cytometry as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
The maximum amount of reagent required to stain 1 x 10e6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
As a general guideline dilutions of 1:50 to 1:200 should be suitable for most applications.
Conjugation PE

Sheep IgG (H+L chain), adsorbed F(ab')2 Fragment donkey polyclonal antibody, PE

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: The maximum amount of reagent required to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
As a general guideline dilutions of 1:50 to 1:200 should be suitable for most applications.
Conjugation PE

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed donkey polyclonal antibody, PE

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Flow Cytometry: the maximum amount of reagent required to stain 1x10e6 cells is approx. 1.0 µg of antibody conjugate.
As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Conjugation PE

Human IgM (Fc specific) mouse monoclonal antibody, clone CH2, PE

Applications Flow cytometry: 0.5 µg/ml.
Reactivities Human
Conjugation PE

Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, PE

Applications Flow Cytometry: Use 50 µl of 1/20 diluted anti body to label 106 cells in 100 µl.
Reactivities Mouse
Conjugation PE

Mouse IgG (H+L chain), adsorbed goat polyclonal antibody, PE

Applications Flow Cytometry: Use 50 µl of 1/10 diluted antibody to label 10e6 cells in 100 µl.
Reactivities Mouse
Conjugation PE

Rat IgG (H+L chain), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Applications Flow cytometry (1/5 - 1/10): use 50 µl of the suggested working dilution to label 10e6 cells in 100 µl.
Reactivities Rat
Conjugation PE