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Guinea Pig IgG (Fc specific) sheep polyclonal antibody, FITC
| Applications | ELISA. (In)direct immunofluorescence. Immunocytochemistry. Immunohistochemistry on Frozen Sections. Can be used in direct staining of cytoplasmic IgG in fixed guinea pig cells and tissue substrates; to identify circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of guinea pig in the middle layer of the test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: 1/20-1/80. |
| Reactivities | Guinea Pig |
| Conjugation | FITC |
| Immunogen | Purified normal IgG isolated from pooled guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
