Goat IgG (H+L chain) chicken polyclonal antibody, HRP
Applications | ELISA: a 1/75,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate. Western Blot (1/5000). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in Agarose gels and ELISA analysis. |
Reactivities | Goat |
Conjugation | HRP |
Immunogen | Purified, non-denatured Goat IgG (containing both heavy and light chains) emulsified in Freund's adjuvant. Production: After multiple injections, eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-goat IgG's were affinity-purified over a column conjugated with purified Goat IgG (containing both heavy and light chains) and then conjugated with Horseradish Peroxidase. |