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Chicken IgM (Fc specific) goat polyclonal antibody, Texas Red
| Applications | In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in chicken serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of chicken IgM from serum or plasma. Recommended Working Dilutions: Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum. In double radial immunodiffusion (Ouchterlony): a rosette arrangement with 10 µl in a 3 mm diameter center well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. Antibody titre: Precipitin titre 1/32 when tested against pooled normal chicken serum in agar-block immunodiffusion titration. |
| Reactivities | Chicken |
| Conjugation | Texas Red |
| Immunogen | Highly purified normal IgM isolated from pooled chicken serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |