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AACS - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.

Format 2 gRNA vectors, 1 linear donor
Donor DNA EF1a-GFP-P2A-Puro
KN406247 is the updated version of KN206247.

Lenti ORF particles, AACS (Myc-DDK-tagged)-Human acetoacetyl-CoA synthetase (AACS), 200ul, >10^7 TU/mL

Vector pLenti-C-Myc-DDK-P2A-Puro
Tag Myc-DDK
Mammalian Cell Selection Puromycin
  • LentiORF®

AACS CRISPRa kit - CRISPR gene activation of human acetoacetyl-CoA synthetase

Format 3gRNAs, 1 scramble ctrl and 1 enhancer vector

qPCR primer pairs and template standards against Homo sapiens gene AACS

Application Plasmid of exact quantity for transcript copy number calculation

3`UTR clone of acetoacetyl-CoA synthetase (AACS) for miRNA target validation

Vector pMirTarget
Mammalian Cell Selection Neomycin
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase

Transient overexpression of AACS (NM_023928) in HEK293T cells paraffin embedded controls for ICC/IHC staining

Transient overexpression of AACS (NM_023928) in HEK293T cells paraffin embedded 4 um sections, controls for ICC/IHC staining, 5 slides per pack

Transient overexpression of AACS (NM_023928) in HEK293T cells paraffin embedded 4 um sections, controls for ICC/IHC staining, 25 slides per pack