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Limk2 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.

Format 2 gRNA vectors, 1 linear donor
Donor DNA EF1a-GFP-P2A-Puro
KN509293 is the updated version of KN309293.

Limk2 (GFP-tagged) - Mouse LIM motif-containing protein kinase 2 (cDNA clone MGC:5783 IMAGE:3496836)

Vector pCMV6-AC-GFP
Tag TurboGFP
Mammalian Cell Selection Neomycin
  • TrueORF®

Limk2 (GFP-tagged) - Mouse LIM motif-containing protein kinase 2 (Limk2) transcript variant 2, (10ug)

Vector pCMV6-AC-GFP
Tag TurboGFP
Mammalian Cell Selection Neomycin
  • TrueORF®

Limk2 (GFP-tagged) - Mouse LIM motif-containing protein kinase 2 (Limk2) transcript variant 1, (10ug)

Vector pCMV6-AC-GFP
Tag TurboGFP
Mammalian Cell Selection Neomycin
  • TrueORF®

Limk2 (Myc-DDK-tagged) - Mouse LIM motif-containing protein kinase 2 (Limk2), transcript variant 3

Vector pCMV6-Entry
Tag Myc-DDK
Mammalian Cell Selection Neomycin
  • TrueORF®

Lenti ORF clone of Limk2 (Myc-DDK-tagged) - Mouse LIM motif-containing protein kinase 2 (Limk2), transcript variant 3

Vector pLenti-C-Myc-DDK-P2A-Puro
Tag Myc-DDK
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF particles, Limk2 (Myc-DDK-tagged) - Mouse LIM motif-containing protein kinase 2 (Limk2), transcript variant 3, 200ul, >10^7 TU/mL

Vector pLenti-C-Myc-DDK-P2A-Puro
Tag Myc-DDK
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF clone of Limk2 (mGFP-tagged) - Mouse LIM motif-containing protein kinase 2 (Limk2), transcript variant 3

Vector pLenti-C-mGFP-P2A-Puro
Tag mGFP
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF particles, Limk2 (GFP-tagged) - Mouse LIM motif-containing protein kinase 2 (Limk2), transcript variant 3, 200ul, >10^7 TU/mL

Vector pLenti-C-mGFP-P2A-Puro
Tag mGFP
Mammalian Cell Selection Puromycin
  • LentiORF®

Limk2 (Myc-DDK-tagged) - Mouse LIM motif-containing protein kinase 2 (Limk2), transcript variant 2

Vector pCMV6-Entry
Tag Myc-DDK
Mammalian Cell Selection Neomycin
  • TrueORF®

Lenti ORF clone of Limk2 (Myc-DDK-tagged) - Mouse LIM motif-containing protein kinase 2 (Limk2), transcript variant 2

Vector pLenti-C-Myc-DDK-P2A-Puro
Tag Myc-DDK
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF particles, Limk2 (Myc-DDK-tagged) - Mouse LIM motif-containing protein kinase 2 (Limk2), transcript variant 2, 200ul, >10^7 TU/mL

Vector pLenti-C-Myc-DDK-P2A-Puro
Tag Myc-DDK
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF clone of Limk2 (mGFP-tagged) - Mouse LIM motif-containing protein kinase 2 (Limk2), transcript variant 2

Vector pLenti-C-mGFP-P2A-Puro
Tag mGFP
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF particles, Limk2 (GFP-tagged) - Mouse LIM motif-containing protein kinase 2 (Limk2), transcript variant 2, 200ul, >10^7 TU/mL

Vector pLenti-C-mGFP-P2A-Puro
Tag mGFP
Mammalian Cell Selection Puromycin
  • LentiORF®

Limk2 (Myc-DDK-tagged) - Mouse LIM motif-containing protein kinase 2 (Limk2), transcript variant 1

Vector pCMV6-Entry
Tag Myc-DDK
Mammalian Cell Selection Neomycin
  • TrueORF®

Lenti ORF clone of Limk2 (Myc-DDK-tagged) - Mouse LIM motif-containing protein kinase 2 (Limk2), transcript variant 1

Vector pLenti-C-Myc-DDK-P2A-Puro
Tag Myc-DDK
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF particles, Limk2 (Myc-DDK-tagged) - Mouse LIM motif-containing protein kinase 2 (Limk2), transcript variant 1, 200ul, >10^7 TU/mL

Vector pLenti-C-Myc-DDK-P2A-Puro
Tag Myc-DDK
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF clone of Limk2 (mGFP-tagged) - Mouse LIM motif-containing protein kinase 2 (Limk2), transcript variant 1

Vector pLenti-C-mGFP-P2A-Puro
Tag mGFP
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF particles, Limk2 (GFP-tagged) - Mouse LIM motif-containing protein kinase 2 (Limk2), transcript variant 1, 200ul, >10^7 TU/mL

Vector pLenti-C-mGFP-P2A-Puro
Tag mGFP
Mammalian Cell Selection Puromycin
  • LentiORF®

Limk2 (untagged) - Mouse LIM motif-containing protein kinase 2 (Limk2), transcript variant 1, (10ug)

Vector PCMV6-Kan/Neo
Tag Tag Free
Mammalian Cell Selection Neomycin

Limk2 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).

Limk2 CRISPRa kit - CRISPR gene activation of mouse LIM motif-containing protein kinase 2

Format 3gRNAs, 1 scramble ctrl and 1 enhancer vector

qPCR primer pairs and template standards against Mus musculus gene Limk2

Application Plasmid of exact quantity for transcript copy number calculation

qSTAR qPCR primer pairs against Mus musculus gene Limk2

Limk2 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector

Format Lentiviral plasmids
Vector pGFP-C-shLenti

Limk2 - Mouse shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Format Lentiviral particles
Vector pGFP-C-shLenti

Limk2 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector

Format Retroviral plasmids
Vector pRS