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Shh (Myc-DDK-tagged) - Mouse sonic hedgehog (Shh)

Vector pCMV6-Entry
Tag Myc-DDK
Mammalian Cell Selection Neomycin
  • TrueORF®

Shh (GFP-tagged) - Mouse sonic hedgehog (Shh), (10ug)

Vector pCMV6-AC-GFP
Tag TurboGFP
Mammalian Cell Selection Neomycin
  • TrueORF®

Shh - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.

Format 2 gRNA vectors, 1 linear donor
Donor DNA EF1a-GFP-P2A-Puro
KN515749 is the updated version of KN315749.

Lenti ORF clone of Shh (Myc-DDK-tagged) - Mouse sonic hedgehog (Shh)

Vector pLenti-C-Myc-DDK-P2A-Puro
Tag Myc-DDK
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF clone of Shh (mGFP-tagged) - Mouse sonic hedgehog (Shh)

Vector pLenti-C-mGFP-P2A-Puro
Tag mGFP
Mammalian Cell Selection Puromycin
  • LentiORF®

Purified recombinant protein of Mouse sonic hedgehog (Shh).

Tag Tag Free
Expression Host E. coli

Shh (untagged) - Mouse sonic hedgehog (Shh), (10ug)

Vector pCMV6-Entry
Tag Tag Free
Mammalian Cell Selection Neomycin

qSTAR qPCR primer pairs against Mus musculus gene Shh

Sonic hedgehog (SHH) (25-198, His-tag) mouse recombinant protein, 0.5 mg

Tag His-tag
Expression Host E. coli

Sonic hedgehog (SHH) (25-198, His-tag) mouse recombinant protein, 0.1 mg

Tag His-tag
Expression Host E. coli

Shh CRISPRa kit - CRISPR gene activation of mouse sonic hedgehog

Format 3gRNAs, 1 scramble ctrl and 1 enhancer vector

Shh (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).

Shh - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector

Format Lentiviral plasmids
Vector pGFP-C-shLenti

Shh - Mouse shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Format Lentiviral particles
Vector pGFP-C-shLenti

Purified recombinant protein of Mouse sonic hedgehog (Shh), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Tag C-MYC/DDK
Expression Host HEK293T

Shh - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector

Format Retroviral plasmids
Vector pRS