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Posts for 2019
The Revival of Lentiviral Vectors
Dec 11, 2019
In the early 1990s, researchers set out to harness viruses for gene therapy applications. The idea was to co-opt a virus’ natural ability to target cells, transverse the membrane and deliver genetic cargo for novel gene-based therapies. But like many early-stage technologies, challenges, setbacks and missteps dampened initial enthusiasm. Lentivirus-based vectors are increasingly used in gene and cell therapy research because theyhave the ability to...
"Where do you get your protein?" Survey Results
Apr 08, 2019
Recently, OriGene conducted a survey in our scientific community on proteins that scientists use in their research. Over 900 scientists responded to the survey worldwide. Below are some of the questions on the survey: Where do you get your proteins? How do you find your proteins? Which of the followings is the top factor you consider when selecting a protein product? What are the downstream...
Tips on Molecular Cloning
Mar 12, 2019
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules. These recombinant molecules are used to direct their replication within host organism (1,2). In this article, we describe the basic molecular cloning technique that helps every scientist in their laboratory on a regular basis. Plasmid based cloning comprises of 4 main steps: Insert DNA preparation Vector...
HSD17B13 (Hydroxysteroid 17-Beta Dehydrogenase 13)
Jan 23, 2019
HSD17B13, a member of a large family of 17β-hydroxysteroid dehydrogenases, gained a sudden fame recently. A genetic study in 2018 discovered that a genetic variant is associated with a reduced risk for chronicle liver diseases, including NAFLD (non-alcoholic fatty liver disease) and NASH (non-alcoholic steatohepatitis). More studies indicate the progress of NASH to HCC can also be reduced by the presence of the variant. Clin...