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Posts for 2023
ASOs vs siRNA – Which is Right for Me?
Dec 21, 2023
Antisense Oligonucleotides (ASOs) and small interfering RNAs (siRNAs) emerge as pivotal tools in molecular biology, offering precise means to silence targeted genes. In this blog we will explore the intricacies of these powerful gene-silencing tools, delving into their mechanisms and key differences. What are Antisense Oligonucleotides? Antisense oligonucleotides (ASOs) are synthetic, single-stranded DNA or RNA molecules designed to bind selectively to complementary RNA sequences. On...
Understanding Plasmid Multimers
Dec 12, 2023
Plasmids are small, circular, double-stranded DNA molecules found in bacteria and some other single-celled organisms like archaea. They exist separate from the chromosomal DNA and carry genes that are often advantageous but not essential for the host cell's survival. Purified plasmids serve as versatile tools in scientific research and practical applications. In research laboratories, they're pivotal for gene cloning, allowing scientists to deliver specific genes...
Unlocking the secrets: A proven guide to mastering ELISA assay troubleshooting
Dec 01, 2023
ELISAs are widely used to quantify or detect antigens, hormones and antibodies. ELISA assays rely on an antibody that’s specific for the target of interest, and detection methods may be direct, two step, or rely on two types of antibodies (capture and detection). When running ELISAs it’s not unusual to encounter various types of assay issues. However, these issues result in reduced accuracy, increased variability,...
Lentivirus Biosafety: Ensuring Safe and Responsible Research
Nov 20, 2023
Lentiviruses have become indispensable tools in molecular biology, enabling precise gene delivery and manipulation. As researchers harness the potential of lentiviral vectors, it is paramount to prioritize biosafety. There have been considerable efforts to improve the safety of lentiviral vectors, thus 3 different generations exist, with increasing safety modifications. 2nd and 3rd generation systems are the most common vectors seen in the marketplace, thus knowing...
Tips and Tricks for troubleshooting common western blot issues
Nov 17, 2023
Western blots are a ubiquitous technique used in research labs to study proteins of interest. Western blotting is used for antibody validation, protein-protein interaction studies, and many other applications.1 However, generating interpretable western blot results can be challenging. Below are some commonly encountered issues and how to resolve them.2 High Background Could be caused by Try this High antibody concentrationSet up a titration with both...
CytoSections™ - Use case for antibody validation of the IDH1(R132H) mutation in Glioblastomas
Nov 09, 2023
Gliomas are the most frequent type of malignant brain tumor in adults, occuring in over 80% of cases.1 The enzyme isocitrate dehydrogenase, (IDH), plays a crucial role in regular metabolic processes and maintaining homeostasis. IDH mutations however, are characteristic of a specific group of diffuse gliomas.2 In particular, mutation of IDH1 is a key indicator for patient survival. Patients with secondary glioblastomas containing the IDH1(R132H)...
7 Important Considerations When Selecting a Protein Expression System
Aug 22, 2023
Selecting the most suitable expression system for your research depends on various factors to ensure success. Whether you're embarking on cutting-edge research or pursuing large-scale biomanufacturing, understanding the considerations involved in selecting an expression system is of utmost importance. When choosing an expression system, you should consider the following factors: Expression Levels: Bacterial systems offer high expression levels, making them suitable for rapid and cost-effective...
Breaking News: FDA Approves ELEVIDYS™ as First Gene Therapy for Duchenne Muscular Dystrophy!
Jun 27, 2023
On June 22nd, 2023, the FDA provided accelerated approval for ELEVIDYS™, the first gene therapy to treat Duchenne Muscular Dystrophy (DMD)2. DMD is a genetic disorder that results in progressive muscle degeneration due to altered expression of the protein dystrophin, which is responsible for strengthening muscle fibers and facilitating safe muscle contractions1,4. Without proper dystrophin expression, muscle cells become fragile and easily damaged. The symptom...
Lentiviral Vectors as Catalysts for Vaccine Development
Jun 16, 2023
In the ever-evolving landscape of genetic research, lentiviral gene delivery has emerged as a powerful tool that has revolutionized the field of gene therapy and vaccine development. Lentiviral vectors (LVs), derived from HIV-1, have gained significant attention due to their unique ability to efficiently and stably transfer genetic material into both dividing and non-dividing cells. Particularly in the realms of infectious diseases and immuno-oncology, lentiviral...
Lentiviral Vectors as Catalysts for Vaccines
Jun 16, 2023
In the ever-evolving landscape of genetic research, lentiviral gene delivery has emerged as a powerful tool that has revolutionized the field of gene therapy and vaccine development. Lentiviral vectors (LVs), derived from HIV-1, have gained significant attention due to their unique ability to efficiently and stably transfer genetic material into both dividing and non-dividing cells. Particularly in the realms of infectious diseases and immuno-oncology, lentiviral...