Villin (VIL1) Mouse Monoclonal Antibody [Clone ID: CWWB1]

CAT#: AM00355SU-N

Villin (VIL1) mouse monoclonal antibody, clone CWWB1, Supernatant

Product Images

Formalin-Fixed, Paraffin-Embedded Human small intestine stained with Villin antibody Cat.-No. AM00355SU-N using peroxidase conjugate and DAB chromogen. Note brush border and cytoplasmic staining of epithelial cells.

Specifications

Product Data

• Clone Name: CWWB1
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/200
  Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
  Use Polymer anti Mouse/Rabbit IgG as a detection system.
  Positive Control: Colon carcinoma, small intestine.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human Villin protein.
• Specificity: This antibody recognizes Villin.
  Cellular Localization: Cytoplasmic.
• Formulation: State: Supernatant
  State: Liquid Tissue Culture Supernatant with 0.2% BSA and 15mM Sodium Azide.
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens PMS1 homolog 2, mismatch repair system component (PMS2), transcript variant 1
• Database Link:
  Entrez Gene 5395 HumanEntrez Gene 7429 Human
• Background: Villin can cap, nucleate, sever and bundle actin in a Ca and phosphoinositide-regulated manner. It is associated with the microvillar actin core bundle of intestinal and renal brush border implicated in adsorption. Villin is composed of six repeats, each containing 150 residues that together constitute the core domain followed by the carboxyl-terminal headpiece domain of 87 residues. The core domain retains the Ca dependent capping nucleating and severing activity, whereas the headpiece domain contributes towards actin filament bundling and binding F-actin, independently of Ca.
• Synonyms: VIL1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Mismatch repair