Thymidylate Synthase (TYMS) Mouse Monoclonal Antibody [Clone ID: TS106]

CAT#: AM05335SU-N

Thymidylate Synthase (TYMS) mouse monoclonal antibody, clone TS106, Supernatant

Product Images

Formalin-Fixed, Paraffin-Embedded Human colon carcinoma stained with Thymidylate Synthase antibody Cat.-No. AM05335SU-N using peroxidase conjugate and DAB chromogen. Note the cytoplasmic staining of tumor cells.

Specifications

Product Data

• Clone Name: TS106
• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot.
  Immunofluorescense.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50
  Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
  Use Polymer anti Mouse/Rabbit IgG as a detection system.
  Positive Control: 5-FU-resistant colon carcinoma or carcinoma cell lines (NCI H630R10, NCI630R1).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human Thymidylate Synthase.
• Specificity: Recognizes Thymidylate synthase (TS).
  Cellular Localization: Cytoplasmic.
• Formulation: State: Supernatant
  State: Liquid Tissue Culture Supernatant with 0.2% BSA and 15mM Sodium Azide.
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens PMS1 homolog 2, mismatch repair system component (PMS2), transcript variant 1
• Database Link:
  Entrez Gene 5395 HumanEntrez Gene 7298 Human
• Background: TS (EC:2.1.1.45), a cytosolic enzyme, is a dimmer of two identical monomers of about 36kDa. The enzyme provides the sole intracellular de novo source of thymidylate and plays a crucial role in DNA replication and repair. TS catalyzes the methylation of deoxyuridine monophosphate (dump) and its conversion to deoxythymidine monophosphate (dTMP). Therefore, TS is primarily active in proliferating and metabolic active cells. TS is a central target of the widely used antineoplastic agent 5-Fluorouracil (5-FU) and thus also of the Xeloda, which is enzymatically activated to 5-FU. TS is inactivated by a covalent complex formation with 5-FdUMP and methylenetetrahydrofolate. Literature indicates that expression of TS is associated with response to 5-fluorouracil (5-FU) in human breast, colorectal, gastric, head, and neck carcinomas with low TS expression predicting better response to 5-FU and survival.
• Synonyms: TYMS, TSase, OK/SW-cl.29

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Mismatch repair