HPRT (HPRT1) Mouse Monoclonal Antibody [Clone ID: AT2G8]

CAT#: AM09392PU-S

HPRT (HPRT1) mouse monoclonal antibody, clone AT2G8, Purified

Product Images

Western blot analysis: The extracts of 293T (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HPRT (1:500-1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: AT2G8
• Applications: ELISA, WB
• Recommended Dilution: ELISA.
  Western blot (1/500-1/5000). Recommended starting dilution is 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human HPRT (1-218aa) purified from E. coli
• Specificity: The antibody recognizes Human HPRT. Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens hypoxanthine phosphoribosyltransferase 1 (HPRT1)
• Database Link:
  Entrez Gene 3251 Human
• Background: HPRT is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. HPRT, which acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP, functions primarily to salvage purines from degraded DNA to renewed purine synthesis.
• Synonyms: HGPRTase, HGPRT

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Drug metabolism - other enzymes, Metabolic pathways, Purine metabolism