PTEN Mouse Monoclonal Antibody [Clone ID: 6H2.1]

CAT#: AM10137SU-N

PTEN mouse monoclonal antibody, clone 6H2.1, Supernatant

Product Images

Formalin-Fixed, Paraffin-Embedded Human prostate stained with PTEN antibody Cat.-No. AM10137SU-N using peroxidase conjugate and AEC chromogen. Note nuclear staining of glandular epithelial cell.

Specifications

Product Data

• Clone Name: 6H2.1
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/100
  Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
  Use Polymer anti Mouse/Rabbit IgG as a detection system.
  Positive Control: Fetal liver or hepatocellular carcinoma.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: C-terminal 100 amino acids of PTEN
• Specificity: Recognizes PTEN.
  Cellular Localization: Nuclear.
• Formulation: State: Supernatant
  State: Liquid purified Ig fraction with 0.2% BSA and 15mM Sodium Azide.
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens PMS1 homolog 2, mismatch repair system component (PMS2), transcript variant 1
• Database Link:
  Entrez Gene 5395 HumanEntrez Gene 5728 Human
• Background: PTEN (phosphatase and tensin homolog deleted on chromosome ten) is a tumor suppressor gene that is mutated in a wide range of cancers. PTEN is located on chromosome sub-band 10q23.3 and encodes a 403 amino acid protein which acts as a dual-specific protein and phospholipid phosphatase. As a lipid phosphatase, PTEN regulates phosphatidylinositol-3’-kinase by dephosphorylating the D3 position of phosphatidylinositol (3,4,5)-triphosphate and phosphatidylinositol (3,4)-biophosphate substrates, thereby antagonizing signal transduction downstream of phosphatidylinositol-(PI-3) kinase.7 Loss-of-function mutations in the PTEN gene lead to constitutive activation of multiple signaling pathways including the PI3K/Akt pathway which affects cell proliferation, apoptosis and migration. PTEN has also been shown to control p53 protein levels and transcriptional activity.
• Synonyms: MMAC1, TEP1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Mismatch repair