Tartrate Resistant Acid Phosphatase (ACP5) Mouse Monoclonal Antibody [Clone ID: 9C5]

CAT#: AM10145SU-N

Tartrate Resistant Acid Phosphatase (ACP5) mouse monoclonal antibody, clone 9C5, Supernatant

Product Images

Formalin-Fixed, Paraffin-Embedded Human bone marrow with hairy cell leukemia stained with TRAcP antibody Cat.-No. AM10145SU-N using peroxidase conjugate and DAB chromogen. Note the cytoplasmic staining of leukemia cells.

Specifications

Product Data

• Clone Name: 9C5
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/200
  Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
  Use Polymer anti Mouse/Rabbit IgG as a detection system.
  Positive Control: Hairy cell leukemia.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Purified Human TRAcP protein.
• Specificity: Recognizes TRAcP.
  Anti-TRAcP antibody labels the cells of hairy cell leukemia (HCL) with a high degree of sensitivity and specificity. Other cells stained with this antibody are tissue macrophages and osteoclasts, which also express abundant TRAcP activity.
  Cellular Localization: Cytoplasmic.
• Formulation: State: Supernatant
  State: Liquid purified Ig fraction with 0.2% BSA and 15mM Sodium Azide.
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens PMS1 homolog 2, mismatch repair system component (PMS2), transcript variant 1
• Database Link:
  Entrez Gene 5395 HumanEntrez Gene 54 Human
• Background: Tartrate-resistant acid phosphatase (TRAP) is a basic iron-binding protein with high activity towards phosphoproteins, ATP and 4-nitrophenyl phosphatase. It is detected in human alveolar macrophages, osteoclasts, spleen and liver. Its expression is increased in spleen and monocytes of patients with Gaucher’s disease; spleenocytes and circulating leukocytes of patients with hairy cell leukemia; spleen of patients with Hodgkin's lymphoma and in the sera of patients undergoing active bone turnover.
  The histochemical identification of hairy cell leukemia via tartrate-resistant acid phosphatase assay has been a standard for over two decades.
• Synonyms: Tartrate-resistant acid phosphatase type 5, TRAP5, TRAcP

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Mismatch repair