ZAP70 Mouse Monoclonal Antibody [Clone ID: 2F3.2]

CAT#: AM10148SU-N

ZAP70 mouse monoclonal antibody, clone 2F3.2, Supernatant

Product Images

Formalin-Fixed, Paraffin-Embedded Human CLL/SLL stained with ZAP70 antibody Cat.-No. AM10148SU-N using peroxidase conjugate and DAB chromogen. Note the cytoplasmic staining of lymphoma cells.

Specifications

Product Data

• Clone Name: 2F3.2
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/200
  Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
  Use Polymer anti Mouse/Rabbit IgG as a detection system.
  Positive Control: Tonsil, CLL/SLL.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: GST-fusion to tandem SH2 domains of Human ZAP-70 corresponding to residues 1-254
• Specificity: This antibody recognizes ZAP-70.
  Cellular Localization: Cytoplasmic.
• Formulation: State: Supernatant
  State: Liquid Tissue Culture Supernatant with 0.2% BSA and 15mM Sodium Azide.
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens PMS1 homolog 2, mismatch repair system component (PMS2), transcript variant 1
• Database Link:
  Entrez Gene 5395 HumanEntrez Gene 7535 Human
• Background: ZAP-70 is a 70 kD protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. ZAP-70 protein is expressed in leukemic cells of approximately 25% of Chronic Lymphocytic Leukemia (CLL) cases as well. ZAP-70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (ZAP-70 negative) and Ig-unmutated (ZAP-70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The ZAP-70 positive Ig-unmutated CLL cases have a poorer prognosis.
• Synonyms: ZAP-70, ZAP 70, SRK

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Mismatch repair