Aurora A (AURKA) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 7F12]

CAT#: AM20207PU-N

Aurora A (AURKA) (N-term) (incl. pos. control) mouse monoclonal antibody, clone 7F12, Purified


USD 465.00

2 Weeks*

Size
    • 100 ug

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Specifications

Product Data
Clone Name 7F12
Applications WB
Recommended Dilution Immunobotting (Western Blot): 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking buffer and blot incubation buffer.
Included Postitive Control: Cell Lysate from untreated A431 cells.
Reactivities Canine, Human, Mouse, Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Peptide conjugated to hemocyanin
Epitope: N-terminus.
Specificity Recognizes Aurora-A (N-term).
Formulation PBS / 0.09% Sodium Azide / PEG and Sucrose.
State: Purified
State: Lyophilized purified IgG fraction.
Reconstitution Method Restore with 1 ml H2O (15 min, RT).
Purification Size Exclusion Chromatography.
Storage Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -20°C to -80°C.
Avoid repeated freezing and thawing.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months.
Stability Shelf life: one year from despatch.
Gene Name Homo sapiens aurora kinase A (AURKA), transcript variant 2
Background Aurora proteins are members of a serine/threonine kinase family. They play a crucial role in mitosis by regulating chromosome segregation and cytokinesis. There are three forms of Aurora proteins in mammalian cells: AuroraA, B and C. Aurora-A (Aurora-2; STK6, ARK1, Aurora/IPL-1 related kinase) associates with centrosomes and microtubules during mitosis. Phosphorylation of a threonine residue within the activation loop of the catalytic domain lead to activation of Aurora-A. AuroraB (Aurora-1) is responsible for chromatin modification and histone H3 phosphporylation.
Synonyms AURKA, AIK1, ARK1, AURA, BTAK, STK15, STK6, Aurora/IPL1-related kinase 1, AURORA2
Note Molecular Weight: 47 kDa

Protocol: Positive Control: Cell Lysate from untreated A431 cells.
Formulation: Lyophilized Cell lysate from Serum starved A431 cells.
Stability:
Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
Application:
The Positive Control lysate is recommended for Immunoblot applications. 20µl of Positive Control correspond to ca. 20.000 cells.
Use 20µl/lane (mini gel) for HRPO/ECL detection of the target proteins.
Storage:
Aliquote and store frozen.
Avoid repeated freeze/thaw cycles.
Shelf life: one year from despatch.
The Lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as Immunoprecipitation.
Reference Data
Protein Families Druggable Genome, Protein Kinase, Stem cell - Pluripotency
Protein Pathways Oocyte meiosis

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.