Aurora A (AURKA) (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5F11]

CAT#: AM20208PU-N

Aurora A (AURKA) (C-term) (incl. pos. control) mouse monoclonal antibody, clone 5F11, Purified

Product Images

Detection of endogenous Aurora-A/B: Whole cell extracts of vanadate treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The Immunoblot was probed with monoclonal Aurora-A/B antibody AM20208PU-N (5F11) (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2: SW480 Lane 3: SW620 Lane 4: HT29 Lane 5: MCF-7 Lane 6: MDA-MB231 Lane 7: T47D

Specifications

Product Data

• Clone Name: 5F11
• Applications: FC, IF, WB
• Recommended Dilution: Immunobotting (Western Blot): 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking buffer and blot incubation buffer.
  Included Postitive Control: Cell lysate from untreated SW620 cells.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Peptide conjugated to hemocyanin
  Epitope: C-terminus.
• Specificity: Recognizes Aurora-A/B (C-term).
• Formulation: PBS / 0.09% Sodium Azide / PEG and Sucrose.
  State: Purified
  State: Lyophilized purified IgG fraction.
• Reconstitution Method: Restore with 1 ml H2O (15 min, RT).
• Purification: Size Exclusion Chromatography.
• Storage: Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -20°C to -80°C.
  Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens aurora kinase A (AURKA), transcript variant 2
• Database Link:
  Entrez Gene 6790 Human
• Background: Aurora proteins are members of a serine/threonine kinase family. They play a crucial role in mitosis by regulating chromosome segregation and cytokinesis. There are three forms of Aurora proteins in mammalian cells: AuroraA, B and C. Aurora-A (Aurora-2; STK6, ARK1, Aurora/IPL-1 related kinase) associates with centrosomes and microtubules during mitosis. Phosphorylation of a threonine residue within the activation loop of the catalytic domain lead to activation of Aurora-A. AuroraB (Aurora-1) is responsible for chromatin modification and histone H3 phosphporylation.
• Synonyms: Aurora kinase A, Aurora A, Aurora B, AURKA, AURKB, AURA, AURB, BTAK, STK6, ARK1, STK15, AIK, AIK2, AIM1, ARK2, STK12
• Note: Molecular Weight: 47 kDa
  
  Protocol: Positive Control: Cell lysate from untreated SW620 cells.
  Formulation: Lyophilized Cell lysate from Serum starved SW620 cells.
  Stability:
  Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
  Application:
  The Positive Control lysate is recommended for Immunoblot applications. 20µl of Positive Control correspond to ca. 20.000 cells.
  Use 20µl/lane (mini gel) for HRPO/ECL detection of the target proteins.
  Storage:
  Aliquote and store frozen.
  Avoid repeated freeze/thaw cycles.
  Shelf life: one year from despatch.
  The Lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as Immunoprecipitation.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Oocyte meiosis