SHMT1 (374-483) Mouse Monoclonal Antibody [Clone ID: 4F9]

CAT#: AM20941PU-N

SHMT1 (374-483) mouse monoclonal antibody, clone 4F9, Purified

Product Images

SHMT1 monoclonal antibody clone 4F9 Western Blot analysis of SHMT1 expression in HeLa.

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  Western Blot analysis of SHMT1 expression in transfected 293T cell line by SHMT1 monoclonal antibody clone 4F9.

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  Detection limit for recombinant GST tagged SHMT1 is approximately 1 ng/ml as a capture antibody.

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  Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

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Specifications

Product Data

• Clone Name: 4F9
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: SHMT1 (NP_004160, 374 a.a. ~ 483 a.a) partial recombinant protein with GST tag
• Specificity: This antibody reacts to Serine Hydroxymethyltransferase, Cytosolic (SHMT1) at 374-483.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A Chromatography
• Storage: Upon receipt, store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens serine hydroxymethyltransferase 1 (SHMT1), transcript variant 1
• Database Link:
  Entrez Gene 6470 Human
• Background: SHMT / SHMT1 is the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. A pseudogene of this gene is located on the short arm of chromosome 1.
• Synonyms: Serine methylase, Glycine hydroxymethyltransferase, Serine hydroxymethyltransferase cytosolic

Reference Data

• Protein Pathways: Cyanoamino acid metabolism, Glycine, serine and threonine metabolism, Metabolic pathways, Methane metabolism, One carbon pool by folate