CBSL Mouse Monoclonal Antibody [Clone ID: 3E1]

CAT#: AM31730PU-N

CBSL mouse monoclonal antibody, clone 3E1, Purified

Product Images

Western Blot analysis of CBS expression in HeLa cell lysate.

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  Western Blot analysis of CBS expression in transfected 293T cell line. Lane 1: CBS transfected lysate (61 KDa). Lane 2: Non-transfected lysate.

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  Western blot analysis of CBS over-expressed 293 cell line, cotransfected with CBS Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CBS antibody, clone 3E1. GAPDH ( 36.1 kDa ) used as specificity and loading control.

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  Detection limit for recombinant GST tagged CBS is approximately 0.1ng/ml as a capture antibody.

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  Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

• Immunoprecipitation of CBS transfected lysate using anti-CBS monoclonal antibody and Protein A Magnetic Bead.

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Specifications

Product Data

• Clone Name: 3E1
• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Immunoprecipitation.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: CBS (NP_000062, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag.
• Specificity: Recognizes Human CBS.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: 0.25 mg/ml
• Purification: Protein A Chromatography
• Gene Name: Homo sapiens cystathionine-beta-synthase (CBS), transcript variant 1
• Database Link:
  Entrez Gene 24250 RatEntrez Gene 102724560 Human
• Background: CBS is involved in transsulfuration pathway. The first step of this pathway, from homocysteine to cystathionine, is catalyzed by CBS. CBS deficiency can cause homocystinuria which affects many organs and tissues including the eyes, the central nervous, skeletal, and vascular systems.
• Synonyms: Cystathionine beta-synthase, Serine sulfhydrase, Beta-thionase

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cysteine and methionine metabolism, Glycine, serine and threonine metabolism, Metabolic pathways, Selenoamino acid metabolism