Karyopherin beta 3 (IPO5) Mouse Monoclonal Antibody [Clone ID: 1C4]

CAT#: AM31807PU-N

Karyopherin beta 3 (IPO5) mouse monoclonal antibody, clone 1C4, Purified

Product Images

Western Blot analysis of IPO5 expression in HeLa cell lysate.

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  Detection limit for recombinant GST tagged IPO5 is approximately 0.03ng/ml as a capture antibody.

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  Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Clone Name: 1C4
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: IPO5 antibody was raised against rANBP5 (AAH01497, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Specificity: This antibody reacts to IPO5 / Importin 5.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage: Store the antibody at -20°C to -80°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens importin 5 (IPO5)
• Database Link:
  Entrez Gene 3843 Human
• Background: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.
• Synonyms: Importin-5, IPO5, Karyopherin beta-3, KPNB3

Reference Data

• Protein Families: Druggable Genome