JNK2 (MAPK9) (1-425) Mouse Monoclonal Antibody [Clone ID: 1C1-3A8]

CAT#: AM31822PU-N

JNK2 (MAPK9) (1-425) mouse monoclonal antibody, clone 1C1-3A8, Purified

Product Images

Western Blot analysis of MAPK9 expression in transfected 293T cell line by MAPK9 monoclonal antibody

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  Western blot analysis of MAPK9 over-expressed 293 cell line, cotransfected with MAPK9 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MAPK9 monoclonal antibody.

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  Detection limit for recombinant GST tagged MAPK9 is approximately 3ng/ml as a capture antibody.

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  Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Clone Name: 1C1-3A8
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: MAPK9 antibody was raised against mAPK9 (AAH32539, 1 a.a. ~ 425 a.a) full length recombinant protein with GST tag.
• Specificity: This antibody reacts to full lenght MAPK9 / JNK2.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens mitogen-activated protein kinase 9 (MAPK9), transcript variant JNK2-g
• Database Link:
  Entrez Gene 5601 Human
• Background: JNK2, a MAPK type protein kinase, regulates cell apoptosis, differentiation and growth in response to growth factors, inflammatory cytokines and cellular stress. JNK2 is phosphorylated by by MKK4/MAP2K4 and MKK7/MAP2K7. Activated JNK2 translocates to the nucleus where it phosphorylates transcription factors, including c-Jun, ATF2, ELK1, Jun-D and NFAT4. At least four distinct isoforms, resulting from alternatively spliced transcript variants, have been reported - three different isoforms comprised of 382 amino acids and one comprised of 424 amino acids.
• Synonyms: JNK-55, PRKM9, MAP kinase 9

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Adipocytokine signaling pathway, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, Focal adhesion, GnRH signaling pathway, Insulin signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, RIG-I-like receptor signaling pathway, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, Wnt signaling pathway