Uromucoid (UMOD) Mouse Monoclonal Antibody [Clone ID: 10.32]

CAT#: AM31843PU-N

Uromucoid (UMOD) mouse monoclonal antibody, clone 10.32, Purified

Specifications

Product Data

• Clone Name: 10.32
• Applications: ELISA, IF, IHC
• Recommended Dilution: Immunofluorescence.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Formalin, Fixed, Paraffin Embedded Sections: 20 µg/ml.
  It can also be used to purify THP by binding to a solid phase.
• Reactivities: Canine, Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human Tamm-Horsfall protein.
• Specificity: Anti-Human Tamm-Horsfall protein (THP) is a monoclonal antibody which reacts with an epitope of the urinary mucoprotein.
  This monoclonal antibody has shown good localization to the distal tubule and collecting duct as well as to large deposits of Tamm-Horsfall protein which has clogged peri-pelvic veins.
• Formulation: PBS containing 0.02% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein G Chromatography.
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens uromodulin (UMOD), transcript variant 2
• Database Link:
  Entrez Gene 7369 Human
• Background: Tamm-Horsfall Protein (THP) is a glycoprotein of approximately 80 kDa containing up to 25% carbohydrate by weight. It exists as a high molecular weight polymer in urine and approximately 50 mg of the THP are excreted per day by adult humans. THP is produced by the epithelial cells lining the thick ascending loop of Henle and distal convoluted tubules of the kidney. THP is also the primary constituent of renal tubular casts and THP aggregation has been implicated as a factor in the pathogenesis of acute renal failure by causing tubular obstruction. Antibody to THP is present in the serum of patients with pyelonephritis and reflus uropathy.
• Synonyms: Tamm-Horsfall urinary glycoprotein, THP