RICTOR (1-99) Mouse Monoclonal Antibody [Clone ID: 1F3]

CAT#: AM31901PU-N

RICTOR (1-99) mouse monoclonal antibody, clone 1F3, Purified

Product Images

Western Blot analysis of RICTOR expression in Hela NE using RICTOR monoclonal antibody clone 1F3.

• 

  Western Blot analysis of RICTOR expression in in transfected 293T cell using RICTOR monoclonal antibody clone 1F3.

• 

  Detection limit for recombinant GST tagged RICTOR is approximately 0.03ng/ml as a capture antibody.

• 

  Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

• 

  Immunoprecipitation of RICTOR transfected lysate using anti-RICTOR monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RICTOR Rabbit polyclonal antibody.

Show all 5 images

Specifications

Product Data

• Clone Name: 1F3
• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Immunoprecipitation.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: RICTOR (NP_689969, 1 a.a. ~ 99 a.a) partial recombinant protein with GST tag
• Specificity: Recognizes RICTOR at aa 1-99.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: 0.42 mg/ml
• Purification: Protein A Chromatography
• Gene Name: Homo sapiens RPTOR independent companion of MTOR complex 2 (RICTOR), transcript variant 1
• Database Link:
  Entrez Gene 253260 Human
• Background: The mTOR pathway (mammalian target of rapamycin) coordinates nutrient signals with growth factor dependent signaling. Recent data revealed that mTOR is organized in two independent protein complexes: The complex containing mTOR, GbL and RAPTOR is target of rapamycin while the complex containing mTOR, GbL and RICTOR is insensitive to rapamycin. The mTOR /RICTOR complex phosphorylates PKBalpha/akt1 at Serine 473
• Synonyms: KIAA1999, Rapamycin-insensitive companion of mTOR, mAVO3

Reference Data

• Protein Pathways: mTOR signaling pathway