Retinol Binding Protein-1 (RBP1) Mouse Monoclonal Antibody [Clone ID: G4E4]

CAT#: AM33316PU-S

Retinol Binding Protein / RBP mouse monoclonal antibody, clone G4E4, Purified

Specifications

Product Data

• Clone Name: G4E4
• Applications: IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Immunofluorescence: 1-2 μg/ml.
  Western Blotting: 0.5-1 μg/ml.
  Immunoprecipitation: 1-2 μg/500 μg protein lysate.
  Immunohistochemistry on Frozen Sections: 1.-2 μg/ml for 30 minutes at RT.
  Positive Control: Liver or placenta.
• Reactivities: Goat, Human, Monkey, Mouse, Rabbit, Rat, Chimpanzee
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Retinol binding protein-1 Purified from human plasma
• Specificity: Cellular Localization: Cytoplasm
• Formulation: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 21-25kDa
• Gene Name: retinol binding protein 1
• Database Link:
  Entrez Gene 5947 Human
  UniProt ID P09455
• Background: This MAb recognizes an epitope within the 74-182 C-terminal sequence (11kD peptide fragment) of human serum Cellular Retinol Binding Protein 1 (CRBP 1), a single-chain glycoprotein belonging to the superfamily of hydrophobic molecule transporter proteins, which is responsible for transport of retinol (vitamin A1) from the liver to peripheral target tissues, like the eye, where it mediates the cellular uptake. CRBP 1 is synthesized by hepatic parenchymal cells where it becomes bound to its ligand retinol and is then released into the circulation, where it binds further to the protein transthyretin, to form a transporting complex, which is big enough not to be lost by filtration through the kidney glomeruli. It is detected in nearly all tissues with higher expression in adult ovary, pancreas, pituitary gland, adrenal gland, and fetal liver.
• Synonyms: Cellular retinol-binding protein I, CRBP1, CRBP2, RBP1, RBP2, RBP4, RBPC, Retinol binding protein 1, Retinol binding protein 1 cellular, Retinol binding protein 2 cellular, Retinol binding protein 4 plasma