BHMT Mouse Monoclonal Antibody [Clone ID: 3D6]

CAT#: AM50001PU-S

BHMT mouse monoclonal antibody, clone 3D6, Purified


USD 230.00

2 Weeks*

Size
    • 50 ul

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Specifications

Product Data
Clone Name 3D6
Applications ELISA, WB
Recommended Dilution ELISA.
Western blot: 1/1000-1/2000. Detects a band of approximately 42 kDa in Mouse liver lysates.
Reactivities Human
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Recombinant Human BHMT (amino acids 1-406) purified from E. coli.
Specificity This antibody detects Betaine Homocysteine Methyltransferase (BHMT), a 45kDa cytosolic enzyme that uses betaine as a methyl donor to catalyze the remethylation of homocysteine to methionine. This remethylation process is essential as it leads to the synthesis of the critical methyl group donor S-adenosylmethionine (SAM), whilst conserving methionine and detoxifying homocysteine.
BHMT is expressed in the liver, kidney, and optic lens. Defects in homocysteine metabolism have a number of adverse side effects, however as yet changes in BHMT function has not been directly linked to them.
Formulation PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
State: Purified
State: Liquid purified Ig fraction
Concentration lot specific
Purification Protein-G Affinity Chromatography
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background Betaine homocysteine methyltransferase (BHMT), a cytosolic enzyme, and its partial fragments were discovered as autolysosomal membrane proteins from rat liver in the presence of leupeptin. BHMT was also found in human liver. BHMT transfers a methyl group from betaine to homocysteine to form DMG (dimethylglycine) and Met. In vivo, liver BHMT expression is influenced by dietary changes in sulphur amino acids, choline and betaine.
Synonyms Betaine--homocysteine S-methyltransferase 1
Reference Data
Protein Pathways Cysteine and methionine metabolism, Glycine, serine and threonine metabolism, Metabolic pathways

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.