BHMT Mouse Monoclonal Antibody [Clone ID: 3D6]

CAT#: AM50001PU-S

BHMT mouse monoclonal antibody, clone 3D6, Purified

Product Images

The extracts of mouse liver (each 20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BHMT (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: 3D6
• Applications: ELISA, WB
• Recommended Dilution: ELISA.
  Western blot: 1/1000-1/2000. Detects a band of approximately 42 kDa in Mouse liver lysates.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant Human BHMT (amino acids 1-406) purified from E. coli.
• Specificity: This antibody detects Betaine Homocysteine Methyltransferase (BHMT), a 45kDa cytosolic enzyme that uses betaine as a methyl donor to catalyze the remethylation of homocysteine to methionine. This remethylation process is essential as it leads to the synthesis of the critical methyl group donor S-adenosylmethionine (SAM), whilst conserving methionine and detoxifying homocysteine.
  BHMT is expressed in the liver, kidney, and optic lens. Defects in homocysteine metabolism have a number of adverse side effects, however as yet changes in BHMT function has not been directly linked to them.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G Affinity Chromatography
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 635 Human
• Background: Betaine homocysteine methyltransferase (BHMT), a cytosolic enzyme, and its partial fragments were discovered as autolysosomal membrane proteins from rat liver in the presence of leupeptin. BHMT was also found in human liver. BHMT transfers a methyl group from betaine to homocysteine to form DMG (dimethylglycine) and Met. In vivo, liver BHMT expression is influenced by dietary changes in sulphur amino acids, choline and betaine.
• Synonyms: Betaine--homocysteine S-methyltransferase 1

Reference Data

• Protein Pathways: Cysteine and methionine metabolism, Glycine, serine and threonine metabolism, Metabolic pathways