MAFK Mouse Monoclonal Antibody [Clone ID: AT2F7]

CAT#: AM50032PU-N

MAFK mouse monoclonal antibody, clone AT2F7, Purified

Product Images

The cell lysates of Jurkat (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAFK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: AT2F7
• Applications: ELISA, WB
• Recommended Dilution: The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity.
  Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
  Recommended starting dilution is 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant human MAFK (1-156aa) purified from E. coli
• Specificity: This antibody recognizes Human MAFK.
  Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Protein-A affinity chromatography
• Gene Name: Homo sapiens MAF bZIP transcription factor K (MAFK)
• Database Link:
  Entrez Gene 7975 Human
• Background: The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locus control regions (LCRs). LCRs are associated with powerful enhancer activity that is mediated by the transcription factor NFE2 (nuclear factor erythroid-2). NFE2 DNA-binding activity consists of a heterodimer containing an 18-kD Maf protein (MafF, MafG or MafK) and p45. Since Maf homodimers lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. The MafK of Maf proteins is primarily expressed during development in mesenchymal and hematopoietic cells and neurons.
• Synonyms: Transcription factor MafK

Reference Data

• Protein Families: Druggable Genome, Transcription Factors