Eosinophil Peroxidase (EPX) Mouse Monoclonal Antibody [Clone ID: EPO104]

CAT#: AM50268PU-S

EPX mouse monoclonal antibody, clone EPO104, Purified

Specifications

Product Data

• Clone Name: EPO104
• Applications: FC, IF, IHC
• Recommended Dilution: ELISA: For coating, order Ab without BSA.
  Flow Cytometry: 0.5-1 µg/million cells.
  Immunofluorescence: 0.5-1 µg/ml.
  Immunohistochemistry on Frozen Sections: 0.5-1.0 µg/ml for 30 minutes at RT.
  Positive Control: MCF-7 cells, Tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human eosinophils from a patient with hypereosinophilic syndrome
• Specificity: Cellular Localization: Cytoplasmic granule
• Formulation: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 70kDa (55kDa-Heavy Chain; 15kDa-Light Chain)
• Gene Name: eosinophil peroxidase
• Database Link:
  Entrez Gene 8288 Human
  UniProt ID P11678
• Background: Peripheral blood granulocytes are classified into neutrophils, basophils and eosinophils according to the staining characteristics of their cytoplasmic granules. Granule proteins are released by physiologic and pharmacologic stimuli and play important roles in both normal and pathological host immune responses. Eosinophil major basic protein and eosinophil peroxidase (EPX) are granule proteins specific to the eosinophil. AHE-1 recognizes human EPX, a granule protein specific to eosinophils. It does not cross-react with eosinophil major basic protein, elastase, cathepsin G, esterase N, thrombin, plasmin, kallikrein, lactoferrin, or transferrin. This MAb stains eosinophils only and does not stain other peripheral blood cells, including platelets, neutrophils, monocytes, lymphocytes or red blood cells. Human EPX gene product can form a tetramer of two light chains and two heavy chains. Other peroxidase family members include myeloperoxidase (MPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO).
• Synonyms: Eosinophil peroxidase heavy chain; EPER; EPO; EPP; EPX; EPX PEN; PERE