Fascin Mouse Monoclonal Antibody [Clone ID: AT13D2]

CAT#: AM50621PU-S

Fascin mouse monoclonal antibody, clone AT13D2, Purified

Product Images

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FSCN1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : HepG2 cell lysate Lane 3. : THP-1 cell lysate

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  ICC/IF analysis of FSCN1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human FSCN1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Specifications

Product Data

• Clone Name: AT13D2
• Applications: ELISA, FC, IF, WB
• Recommended Dilution: The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant human FSCN1 (1-493aa) purified from E. coli.
• Formulation: Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Protein-A affinity chromatography
• Gene Name: Homo sapiens fascin actin-bundling protein 1 (FSCN1)
• Database Link:
  Entrez Gene 6624 Human
• Background: FSCN1 is an actin-bundling protein that provides rigidity to filopodial bundles to efficiently push the membrane forward during cytoskeleton remodeling and cell migration. Probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers. And this protein is absent from most normal epithelia yet is upregulated in multiple forms of human carcinoma, where its expression correlates clinically with a poor prognosis. FSCN1 expression has been localized to neurons, glial cells, and endothelial cells.
• Synonyms: SCN1, FAN1, HSN, SNL, p55, Singed-like protein, Dendritic cell marker