FUBP1 Mouse Monoclonal Antibody [Clone ID: AT14F5]

CAT#: AM50629PU-N

FUBP1 mouse monoclonal antibody, clone AT14F5, Purified

Product Images

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FUBP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: HepG2 cell lysate Lane 3.: Jurkat cell lysate

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  ICC/IF analysis of FUBP1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human FUBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Specifications

Product Data

• Clone Name: AT14F5
• Applications: ELISA, IF, WB
• Recommended Dilution: The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant human FUBP1 (279-448aa) purified from E.coli.
• Formulation: Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Protein-A affinity chromatography
• Gene Name: Homo sapiens far upstream element binding protein 1 (FUBP1), transcript variant 2
• Database Link:
  Entrez Gene 8880 Human
• Background: FUBP1 is a transcriptiol regulator and fulfills an important function in the precise control of c-myc transcription. The c-Myc protein is a transcription factor which regulates the transcription of many different target genes that play a role in proliferation, cell cycle progression, differentiation, apoptosis and cell metabolism. Consequently, FUBP1 is also involved in the regulation of proliferation and differentiation, as confirmed by different experimental approaches. Knockdown of FUBP1 or expression of a domint-negative FUBP1 (D-binding domain lacking effector activity) led to proliferation arrest in U2OS and Saos-2 osteosarcoma cells due to reduced c-myc expression.
• Synonyms: FUSE-binding protein 1, FBP, DNA helicase V, HDH V

Reference Data

• Protein Families: Stem cell - Pluripotency, Transcription Factors