ID4 (N-term) Rabbit Polyclonal Antibody

CAT#: AP01280PU-N

ID4 (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis with extracts from HepG2 cells using ID4 / BHLHB27 antibody.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids N-terminus of Human Id4.
• Specificity: This antibody detects endogenous levels of ID4 / BHLHB27 protein (region surrounding Ser5).
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% Sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 17 kDa
• Gene Name: inhibitor of DNA binding 4, HLH protein
• Database Link:
  Entrez Gene 3400 Human
  UniProt ID P47928
• Background: Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate the expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins (encoded by Id-related genes) also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins (referenced from entrez gene).
• Synonyms: DNA-binding protein inhibitor ID-4, Inhibitor of DNA binding 4