STIM2 Rabbit Polyclonal Antibody

CAT#: AP05701PU-N

STIM2 rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of whole cell lysate from A20 mouse B-cell lymphoma cells probed with Rabbit anti Human STIM2 at 0.5 (A), 1 (B) and 2 (C) ug/ml

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  Formalin fixed, paraffin embedded human spleen stained with Rabbit anti Human STIM2 following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 0.5 - 1.0 µg/ml; detects a band of approximately 105kDa in A20 cell lysates.
  Immunohistochemistry on paraffin sections (requires antigen retrieval using heat treatment prior to staining).
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A 17 amino acid peptide located near the centre of human STIM2.
• Specificity: This antibody recognises an internal region of stromal interaction molecule-2 (STIM2), a ubiquitously expressed type I membrane protein, of which at least three isoforms have been identified.
• Formulation: Phosphate buffered saline containing 0.02% Sodium Azide
  State: Purified
  State: Liquid purified IgG
• Concentration: 1.0 mg/ml
• Gene Name: Homo sapiens stromal interaction molecule 2 (STIM2), transcript variant 2
• Database Link:
  Entrez Gene 116873 MouseEntrez Gene 117087 RatEntrez Gene 57620 Human
• Background: In T-lymphocytes and other haematopoietic cells, the mechanism for increasing intracellular Ca2+, following antigen mediated T-cell activation, is through the store-operated Ca2+ release-activated Ca2+ (CRAC) channels. STIM1 is reported to activate CRAC channels upon Ca2+ depletion. Whereas, STIM2 acts to inhibit the STIM1-mediated store-operated Ca2+ entry, and forms complexes with STIM1, suggesting that these molecules may play a co-ordinated role in controlling Ca2+ entry.
• Synonyms: Stromal interaction molecule 2, KIAA1482