MAP3K8 Rabbit Polyclonal Antibody

CAT#: AP06071PU-N

MAP3K8 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of COT antibody at 1/500 dilution Lane 1:HEK293T cell lysate Lane 2:Mouse brain tissue lysate Lane 3:Rat liver tissue lysate

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  Immunohistochemical (IHC) analysis in paraffin-embedded human brain tissue using MAP3K8 / TPL-2 antibody.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 250-300 of Human Cot.
• Specificity: This antibody detects endogenous levels of MAP3K8 / TPL-2 protein (region surrounding Pro284).
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (>95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~53 kDa
• Gene Name: Homo sapiens mitogen-activated protein kinase kinase kinase 8 (MAP3K8), transcript variant 1
• Database Link:
  Entrez Gene 26410 MouseEntrez Gene 116596 RatEntrez Gene 1326 Human
• Background: The role of mitogen-activated protein kinases (MAPKs) in cell signaling pathways is well established. The rat gene Tpl-2, for tumor progression locus 2, and the human and mouse homologues c-Cot, for cancer osaka thyroid oncogene, encode a proto-oncogene serine/threonine protein kinase that was shown to play a role in the functional activation of the MAP kinase pathway. Overexpression of Cot induces MAP kinase activation in COS-1 and NIH/3T3 cells. Cot-mediated activation of MAP kinase is inhibited by both Ras N17, a dominant negative mutant of c-H-Ras, and Raf-1s621A, a dominant negative mutant of Raf-1, suggesting that Cot functions upstream of Ras and Raf-1.Other studies have shown that a kinase-negative, dominant negative mutant of Cot partially blocks Ras or Raf-1-induced MAP kinase activation, arguing that Cot functions downstream of Ras and Raf-1. To explain these contrasting findings, it has been suggested that Cot, Ras and Raf-1 may form a multimeric complex that phosphorylates MEK-1. Cot has also been shown to be implicated in T lymphocyte activation.
• Synonyms: MAPK, Tumor progression locus 2, COT, ESTF

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway, T cell receptor signaling pathway, Toll-like receptor signaling pathway