CYP24A1 Rabbit Polyclonal Antibody

CAT#: AP06772PU-N

CYP24A1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analyzes of CYP24A1 antibody in extracts from HUVEC cells.

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  Immunohistochemistry (IHC) analyzes of CYP24A1 antibody in paraffin-embedded human prostate carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 400-450 of Human CYP24A1.
• Specificity: This antibody detects endogenous levels of CYP24A1 protein.
  (region surrounding Arg439)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 38 kDa
• Gene Name: Homo sapiens cytochrome P450 family 24 subfamily A member 1 (CYP24A1), transcript variant 1
• Database Link:
  Entrez Gene 1591 Human
• Background: P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds. P450 enzymes can be classified, based on their sequence similarities, into distinct subfamilies, which include CYP1A and CYP2A. The P450 family member, CYP19, catalyzes the conversion of C19 steroids to estrogens in various tissues, including placenta, gonads, adipose tissue, skin and brain. P450 cholesterol 7a-hydroxylase, CYP7A1, is the rate limiting enzyme of bile acid synthesis in the liver, and its expression is mediated by the bile acid receptor FXR. CYP27A1 catalyzes vitamin D 25-hydroxylation and is localized to the mitochondria in kidney and liver. Overexpression of CYP24 (encoding vitamin D 24 hydroxylase) is likely to lead to abrogation of growth control mediated by vitamin D.
• Synonyms: CYP24, Cytochrome P450 24A1, Vitamin D3 24-hydroxylase, 24-OHase, Cytochrome P450-CC24

Reference Data

• Protein Families: Druggable Genome