alpha 1 Fetoprotein (AFP) Rabbit Polyclonal Antibody

CAT#: AP10421PU-N

alpha 1 Fetoprotein (AFP) rabbit polyclonal antibody, Purified

Product Images

Formalin-Fixed, Paraffin-Embedded Human hepatocellular carcinoma stained with AFP antibody Cat.-No. AP10421PU-N using peroxidase conjugate and DAB chromogen. Note cytoplasmic staining of tumor cells.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/150
  Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
  Use Polymer anti Mouse/Rabbit IgG as a detection system.
  Positive Control: Fetal liver or hepatocellular carcinoma.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Human Alpha-Fetoprotein purified from Human cord serum
• Specificity: Recognizes Alpha-fetoprotein (AFP).
  Cellular Localization: Cytoplasmic.
• Formulation: State: Purified
  State: Liquid purified Ig fraction with 0.2% BSA and 15mM Sodium Azide.
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Mus musculus forkhead box P3 (Foxp3), transcript variant 1
• Database Link:
  Entrez Gene 50943 HumanEntrez Gene 174 Human
• Background: This is a major plasma protein produced by the yolk sac and the liver during fetal life. alpha Fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha Fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha Fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. alpha Fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha Fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
  Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasm, especially yolk sac tumors.
• Synonyms: Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP