Pea3 (ETV4) (C-term) Rabbit Polyclonal Antibody

CAT#: AP17352PU-N

Pea3 (ETV4) (C-term) rabbit polyclonal antibody

Product Images

(LEFT) Western blot analysis of ETV4 antibody (C-term) in mouse heart tissue lysates (35 ug/lane). ETV4 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of ETV4 (arrow) using ETV4 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ETV4 gene (Lane 2).

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  Formalin-fixed and paraffin-embedded human brain tissue reacted with ETV4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  Flow cytometric analysis of widr cells using ETV4 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.

• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 426~455 amino acids from the C-terminal region of Human ETV4.
• Specificity: This antibody detects ETV4 at C-term.
• Formulation: PBS
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 53938 Da
• Gene Name: Homo sapiens ETS variant 4 (ETV4), transcript variant 2
• Database Link:
  Entrez Gene 18612 MouseEntrez Gene 2118 Human
• Background: ETV4 is a transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'.
• Synonyms: E1AF, E1A-F

Reference Data

• Protein Families: Druggable Genome, Transcription Factors