c-Myb (MYB) (557-569) Goat Polyclonal Antibody

CAT#: AP20174PU-N

c-Myb (MYB) (557-569) goat polyclonal antibody, Aff - Purified

Product Images

Western blot analysis: MYB antibody staining of Jurkat lysate at 0.5 ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Peptide ELISA: Detection Limit: 1/8000.
  Western blot: 0.5-1.5 µg/ml.
  Detects a band of Approx 85 kDa in lysates of cell line Jurkat.
• Reactivities: Bovine, Canine, Human, Mouse, Porcine, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide corresponding to internal region of Human c-Myb
• Specificity: This antibody is expected to recognize all reported isofoms (NP_001123645.1; NP_005366.2; NP_001123644.1; NP_001155128.1; NP_001155129.1; NP_001155130.1; NP_001155131.1; NP_001155132.1).
• Formulation: Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer
  State: Aff - Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens v-myb avian myeloblastosis viral oncogene homolog (MYB), transcript variant 2
• Database Link:
  Entrez Gene 17863 MouseEntrez Gene 4602 Human
• Background: Myb is a transcriptional activator; DNA-binding protein that recognizes the sequence 5'-YAAC[GT]G-3'. It participates in the control of proliferation and differentiation of hematopoietic progenitor cells. Myb is a transcription factor related to Myc, and is expressed predominantly in haematopoietic cells and immature gastroepithelial cells. TRAF7 stimulates the sumoylation of Myb at Lys-523 and Lys-499, which are the same sites as those used for PIASy-induced sumoylation. A correlation has been established between reduction in sumoylation of Myb and increase in transcriptional activation. Negative influence on transactivation properties by the negative regulatory domain region of c-Myb depends on upon sumoylation.
• Synonyms: c-Myb
• Note: Calculated Molecular Weight: 85.5kDa according to NP_001123645.1).

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transcription Factors