AKAP1 Rabbit Polyclonal Antibody

CAT#: AP20535PU-N

AKAP1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of AKAP149 Antibody (Cat.-No AP20535PU-N) in extracts from HUVEC cells.

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  Immunohistochemistry analysis of AKAP149 Antibody (Cat.-No AP20535PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution:

  Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.

• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of AKAP149 protein.
  (region surrounding asn306)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (>95% pure by SDS-PAGE
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 90 kDa
• Gene Name: Homo sapiens A-kinase anchoring protein 1 (AKAP1), transcript variant 1
• Database Link:
  Entrez Gene 8165 Human
  UniProt ID Q92667
• Background: The type II cAMP-protein kinase (PKA) is a multifunctional kinase with a broad range of substrates. Specificity of PKA signaling is thought to be mediated by the compartmentalization of the kinase to specific sites within the cell. To maintain this specific localization, the R subunit (RII) of PKA interacts with specific RII-anchoring proteins. This family of proteins has been designated A-kinase anchoring proteins (AKAP). Members of this family, including MAP2 (microtubule-associated protein 2), neuronally expressed AKAP 79 and AKAP 150, and the DNA binding AKAP 95, display differential tissue specificity and localization. Evidence suggests that AKAP 79 and AKAP 150 are both capable of anchoring PKA to postsynaptic densities (PSD), which are a network of proteins located on the internal surfaces of excitatory synapses.
• Synonyms: AKAP149, PRKA1, AKAP-1

Reference Data

• Protein Families: Druggable Genome, Transmembrane