GAL4 (LGALS4) Rabbit Polyclonal Antibody

CAT#: AP20547PU-N

GAL4 (LGALS4) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Galectin-4 antibody (Cat.-No.: AP20547PU-N) in extracts from COLO cells.

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  Immunohistochemistry (IHC) analyzes of Galectin-4 antibody (Cat.-No.: AP20547PU-N) in paraffin-embedded human liver carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Galectin-4 protein.
  (region surrounding Lys83)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 36 kDa
• Gene Name: Homo sapiens galectin 4 (LGALS4)
• Database Link:
  Entrez Gene 3960 Human
• Background: Galectins are a family of soluble beta-galactoside-binding animal lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) interactions and play a role in tumor progression, pre-mRNA splicing and apoptosis. One member of this family, Galectin-4, also known as Gal-4, L36 or LGALS4 maps to human chromosome 19q13.13 and encodes a 36-37 kDa protein. The Galectin-4 protein is composed of 323 amino acids and contains two homologous carbohydrate recognition domains (CRD) and all amino acids typically conserved in the galectin family. Expression of Galectin-4 correlates with the malignant potential of human hepatocellular carcinoma (HCC) and is differentially regulated depending on cell-cell contact, serum growth factors, cell growth and cell differentiation status. Galectin-4 expression is detected in epithelial cells of the colon, rectum, intestine, and in HT29 and LS174T cell lines. Galectin-4 is underexpressed in colorectal cancer and is preferentially upregulated in cells prone to peritoneal dissemination.
• Synonyms: LGALS4, Galectin4, Lactose-binding lectin 4, L-36 lactose-binding protein, L36LBP, Antigen NY-CO-27